resumo
Bioimprinting is an easy, sustainable and low-cost technique that promotes a printing of potential substrates on enzyme structure, inducing a more selective and stable conformation. Bioimprinting promotes conformational changes in enzymes, resulting in better catalytic performance. In this work, the effect of bioimprinting ofBurkholderia cepacialipase (BCL) and porcine pancreatic extracts (PPE) with four different fatty acids (lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), and stearic acid (C18:0)) was investigated. The results demonstrated that the better bioimprinting effect was in BCL with lauric acid in esterification reaction, promoting BCL activation in which relative enzyme activity was 70 times greater than nonimprinted BCL. Bioimprinting results were influenced by the carbon chain length of fatty acids imprinted in the BCL, in which the effects were weaker with the chain increase. Molecular docking was performed to better understand the bioimprinting method. The results of these simulations showed that indeed all fatty acids were imprinted in the active site of BCL. However, lauric acid presented the highest imprinting preference in the active site of BCL, resulting in the highest relative activity. Furthermore, Fourier transform infrared (FTIR) analysis confirmed important variations in secondary structure of bioimprinting BCL with lauric acid, in which there was a reduction in the alpha-helix content and an increase in the beta-sheet content that facilitated substrate access to the active site of BCL and led higher rigidity, resulting in high activity. Bioimprinted BCL with lauric acid showed excellent operational stability in esterification reaction, maintaining its original relative activity after five successive cycles. Thus, the results show that bioimprinting of BCL with lauric acid is a successful strategy due to its high catalytic activity and reusability.
palavras-chave
BURKHOLDERIA-CEPACIA LIPASE; CANDIDA-RUGOSA LIPASE; INTERFACIAL ACTIVATION; IONIC LIQUIDS; CATALYZED SYNTHESIS; IMMOBILIZED LIPASE; CRYSTAL-STRUCTURE; OPEN CONFORMATION; ESTERIFICATION; PERFORMANCE
categoria
Biotechnology & Applied Microbiology; Food Science & Technology
autores
Brandao, LMD; Barbosa, MS; Souza, RL; Pereira, MM; Lima, AS; Soares, CMF
nossos autores
Matheus M. Pereira
Investigadoragradecimentos
Fundacao de Apoio a Pesquisa e a Inovacao Tecnologica do Estado de Sergipe; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico