Proteinaceous Hydrogels for Bioengineering Advanced 3D Tumor Models
authors Blanco-Fernandez, B; Gaspar, VM; Engel, E; Mano, JF
nationality International
journal ADVANCED SCIENCE
author keywords 3D in vitro models; cancers; hydrogels; peptides; proteins
keywords SELF-ASSEMBLING PEPTIDE; IN-VITRO MODEL; 3-DIMENSIONAL CELL-CULTURE; BREAST-CANCER CELLS; EXTRACELLULAR-MATRIX; HEPATOCELLULAR-CARCINOMA; DRUG DISCOVERY; CROSS-LINKING; COLLAGEN I; BIOINSPIRED HYDROGELS
abstract The establishment of tumor microenvironment using biomimetic in vitro models that recapitulate key tumor hallmarks including the tumor supporting extracellular matrix (ECM) is in high demand for accelerating the discovery and preclinical validation of more effective anticancer therapeutics. To date, ECM-mimetic hydrogels have been widely explored for 3D in vitro disease modeling owing to their bioactive properties that can be further adapted to the biochemical and biophysical properties of native tumors. Gathering on this momentum, herein the current landscape of intrinsically bioactive protein and peptide hydrogels that have been employed for 3D tumor modeling are discussed. Initially, the importance of recreating such microenvironment and the main considerations for generating ECM-mimetic 3D hydrogel in vitro tumor models are showcased. A comprehensive discussion focusing protein, peptide, or hybrid ECM-mimetic platforms employed for modeling cancer cells/stroma cross-talk and for the preclinical evaluation of candidate anticancer therapies is also provided. Further development of tumor-tunable, proteinaceous or peptide 3D microtesting platforms with microenvironment-specific biophysical and biomolecular cues will contribute to better mimic the in vivo scenario, and improve the predictability of preclinical screening of generalized or personalized therapeutics.
publisher WILEY
isbn 2198-3844
year published 2021
volume 8
issue 4
digital object identifier (doi) 10.1002/advs.202003129
web of science category 38
subject category Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
unique article identifier WOS:000604284400001

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