resumo
Antibody technologies are the most representative success-case in the biopharmaceutical industry. Widely available purification technologies fail in providing a dedicated universal purification platform that can accommodate antibodies structural diversity, namely antibodies from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration from natural and engineered antibody-binding ligands, to rationally design affinity adsorbents able to capture full-length antibodies and fragments. The one-pot Petasis and Ugi combinatorial reactions were sequentially employed to rapidly generate a library of putative solid-phase adsorbents. The best performing adsorbent yielded a single-step recovery, under mild conditions, of human and chicken whole antibodies, antigen-binding fragments and engineered single-domain antibodies from different complex feedstocks. Due to its simple preparation, the lead antibody adsorbent finds broad applicability as a universal purification platform to increase the availability of antibody technologies in research and development.
palavras-chave
PEPTIDE LIGAND
categoria
Engineering, Chemical
autores
Matos, MJB; Trovao, F; Goncalves, J; Rothbauer, U; Freire, MG; Barbosa, AMJB; Pina, AS; Roque, ACA
nossos autores
Mara Guadalupe Freire Martins
Investigador Coordenadoragradecimentos
The authors thank Fundacao para a Ciencia e Tecnologia (FCT) for funding through projects PTDC/BII-BIO/28878/2017 (co-financed by ERDF under the PT2020 Partnership Agreement LISBOA-01-0145FEDER-028878), and fellowships PD/BD/128251/2016 from MIT-PT PhD Bioengineering Systems to MJBM and SFRH/BPD/97585/2013 to ASP and SFRH/BPD/112543/2015 to AJMB. This work was supported by the Applied Molecular Biosciences Unit -UCIBIO (UIDP/04378/2020 and UIDB/04378/2020) and CICECO-Aveiro Institute of Materials (UIDB/50011/2020 and UIDP/50011/2020), which are financed by national funds from FCT.