A purification platform for antibodies and derived fragments using a de novo designed affinity adsorbent
authors Matos, MJB; Trovao, F; Goncalves, J; Rothbauer, U; Freire, MG; Barbosa, AMJB; Pina, AS; Roque, ACA
nationality International
author keywords Antibody purification; Affinity; Capture; Antibody fragments; synthetic ligands
abstract Antibody technologies are the most representative success-case in the biopharmaceutical industry. Widely available purification technologies fail in providing a dedicated universal purification platform that can accommodate antibodies structural diversity, namely antibodies from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration from natural and engineered antibody-binding ligands, to rationally design affinity adsorbents able to capture full-length antibodies and fragments. The one-pot Petasis and Ugi combinatorial reactions were sequentially employed to rapidly generate a library of putative solid-phase adsorbents. The best performing adsorbent yielded a single-step recovery, under mild conditions, of human and chicken whole antibodies, antigen-binding fragments and engineered single-domain antibodies from different complex feedstocks. Due to its simple preparation, the lead antibody adsorbent finds broad applicability as a universal purification platform to increase the availability of antibody technologies in research and development.
publisher ELSEVIER
issn 1383-5866
isbn 1873-3794
year published 2021
volume 265
digital object identifier (doi) 10.1016/j.seppur.2021.118476
web of science category 10
subject category Engineering, Chemical
unique article identifier WOS:000634724800002
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journal impact factor 5.774
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