Extraction and Characterization of beta-Viginin Protein Hydrolysates from Cowpea Flour as a New Manufacturing Active Ingredient

resumo

The increased mortality rates associated with antibiotic resistance has become a significant public health problem worldwide. Living beings produce a variety of endogenous compounds to defend themselves against exogenous pathogens. The knowledge of these endogenous compounds may contribute to the development of improved bioactive ingredients with antimicrobial properties, useful against conventional antibiotic resistance. Cowpea is an herbaceous legume of great interest due to its high protein content and high productivity rates. The study of genetic homology of vicillin (7S) from cowpea (Vigna unguiculata L.) with vicilins from soybean and other beans, such as adzuki, in addition to the need for further studies about potential biological activities of this vegetable, led us to seek the isolation of the vicilin fraction from cowpea and to evaluate the potential in vitro inhibitory action of pathogenic microorganisms. The cowpea beta viginin protein was isolated, characterized, and hydrolyzed in silico and in vitro by two enzymes, namely, pepsin and chymotrypsin. The antimicrobial activity of the protein hydrolysate fractions of cowpea flour was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa, confirming the potential use of the peptides as innovative antimicrobial agents.

palavras-chave

UNGUICULATA L. WALP; VIGNA-UNGUICULATA; ANTIMICROBIAL PEPTIDES; IN-SILICO; CONGLYCININ; RESISTANCE; VITRO

categoria

Engineering

autores

Almeida, TS; Souza, CAD; Silva, MBDE; Batista, FPR; Ferreira, ES; Santos, ALS; Silva, LN; Melo, CR; Bani, C; Bianconi, ML; Cardoso, JC; De Albuquerque-Junior, RLC; Barbosa, RD; Pereira, MM; Souto, EB; Soares, CMF; Severino, P

nossos autores

agradecimentos

This research was funded by CNPq, FAPERJ and CAPES (financial code-001), and by the Fundacao Carolina (Movilidad de profesoradoBrasil-Espana, Movilidad. Estancias de Investigacion, C.2020) granted to P.S.

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