resumo
Propolis is highly diverse and has various health benefits for humans. However, standardizing propolis-based products for use in the pharmaceutical and food industries is challenging. Efficient extraction processes are essential to maximize their biological potential. Ethanol is commonly used as a solvent but has several drawbacks, such as residual flavor and limitations for some individuals. Water is an alternative, but it is not as effective as ethanol. In this context, new strategies are being explored to find an alternative to ethanol and improve water's performance as a solvent. To this end, a new extraction platform of phenolic compounds from Brazilian green propolis using eutectic solvents was developed. Betaine: citric acid (1:2) with 50 wt % of water was found to be the most efficient solvent, with a yield of extraction around 100 mg(polyphenol) g(propolis) (-1) under optimum conditions with an ultrasound-assisted extraction approach (UAE). In vitro biological assays were performed to evaluate the potential of this new extract, mainly regarding antioxidant, antihypertensive, lipase inhibition, and antimicrobial activity. Our results indicate that the eutectic solvent selected in the extraction process enhances the biological potential of the green propolis extract, opening the doors for the development of semi-finished products active against inflammation biological pathways.
palavras-chave
ANTIOXIDANT ACTIVITY; ASSISTED EXTRACTION
categoria
Chemistry; Science & Technology - Other Topics; Engineering
autores
Contieri, L; Ribeiro, TB; Sosa, FHB; Vaz, BMC; Pizani, RS; Pintado, M; Ventura, SPM; Mesquita, LMD; Rostagno, MA
nossos autores
Filipe Hobi Bordon Sosa
Investigador Júnior
Leonardo Mendes de Sousa Mesquita
Bolseiro de pós-Doutoramento
Sónia Patrícia Marques Ventura
Professor AuxiliarProjectos
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was supported by "Fundacao de Amparo a` Pesquisa do Estado de Sao Paulo-FAPESP" through the projects (2018/14582-5 and 2019/13496-0) and fellowships (L.S.C.: 2020/03623-2; L.M.D.S.M.: 2020/08421-9, 2021/11022-1; 2021/11023-8; and R.S.P.: 2020/04067-6). This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brazil (CAPES)- Process 88887.310558/2018-00 and Finance Code 001. M.A.R. thanks the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq for the productivity grants (302610/2021-9). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/ 2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). This work was developed at CBQF-Centro de Biotecnologia e Quimica Fina under the scope of the strategic funding of UIDB/50016/ 2020 unit, financially supported by the Foundation for Science and Technology (FCT).