resumo
The salt effect on the solubility of the amino acids L-aspartic acid, L-glutamic acid, L-tryptophan, and L-tyrosine, seldomly found in the literature, was studied at 298.2 K, in aqueous solutions of KCl and (NH4)(2)SO4, for salt concentrations up to 2.0 mol.kg(-1). In this concentration range, both salts are salting-in agents for glutamic acid and aspartic acid, with a stronger effect induced by (NH4)(2)SO4. Regarding the two aromatic amino acids, a slight increase in the solubility was obtained at low salt concentrations, followed by a stronger salting-out effect, more pronounced by (NH4)(2)SO4 than by KCl. The relative solubility data obtained in this work were compared to literature data for other amino acids in the same electrolyte solutions to establish a relative solubility ranking connected to their structure. Finally, the solubility data were modeled using the electrolyte Perturbed-Chain Statistical Association Theory (ePC-SAFT). The modeling requires parameters for the amino acids and ions as well as melting properties of the amino acids. All these parameters and properties were obtained from previous works. To quantitatively describe the solubility of amino acids upon salt addition, binary interaction parameters (k(ij)) between any amino acid and anions were determined, while between any amino acid and the cations were fixed to k(ij) = 0.08. The k(ij) parameters between amino acid and the inorganic anions show very similar values for amino acids of the same chemical class (e.g. k(ij) between anion and amino acid with apolar side chains), which may be used to systematically reduce the number of adjustable parameters in future work.
palavras-chave
AQUEOUS-ELECTROLYTE SOLUTIONS; L-TYROSINE; THERMODYNAMIC PROPERTIES; MODELING SOLUBILITY; MELTING PROPERTIES; CHLORIDE SOLUTIONS; ASPARTIC ACID; L-LEUCINE; L-CYSTINE; NACL
categoria
Thermodynamics; Chemistry
autores
Aliyeva, M; Brandao, P; Gomes, JRB; Coutinho, JAP; Held, C; Ferreira, O; Pinho, SP
nossos autores
José R. B. Gomes
Investigador Principal
Paula Cristina Ferreira da Silva Brandão
Investigador AuxiliarGrupos
G1 - Materiais Porosos e Nanossistemas
G4 - Materiais Renováveis e Economia Circular
G6 - Materiais Virtuais e Inteligência Artificial
Projectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was developed within the scope of the project CIMOMountain Research Center, UIDB/00690/2020 and LA/P/0007/2020, and CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020, financed by national funds through the Portuguese Foundation for Science and Technology (FCT)/MCTES. Mehriban Aliyeva thanks FCT and European Social Fund (ESF) for her Ph.D. grant (SFRH/BD/139355/2018). The authors also thank Carina Silva for participating in the measurement of part of the solubility data.