resumo
A unique sustained drug-controlled release system was designed by robocasting using a composite ink able to fabricate scaffolds that embrace functions such as local drug delivery and bone tissue regeneration. Polylactic-coglycolic acid nanoparticles loaded with levofloxacin and ibuprofen were successfully developed with a high drugloading capacity, allowing a sustained release of both drugs. The drug-loaded nanoparticles were successfully added to a calcium phosphate-based composite ink, keeping their structural integrity during the printing process. A controlled drug release profile was verified for the composite scaffolds containing encapsulated drug nanoparticles, contrarily to the completely released in the first hours for both scaffolds that contained nonencapsulated drugs (levofloxacin and ibuprofen). The work developed introduces an innovative drug-loaded extrudable feedstock to fabricate bone-like scaffolds as drug delivery systems, not requiring any postprocessing steps.
palavras-chave
PLGA NANOPARTICLES; BONE REGENERATION; MOLECULAR-WEIGHT; IN-VITRO; LEVOFLOXACIN; ENCAPSULATION; DESIGN; DOSAGE
categoria
Pharmacology & Pharmacy
autores
Soares, F; Ribeiro, N; Baia, A; Torres, PMC; Sarmento, B; Olhero, SM
nossos autores
Nilza Maria Lopes Ribeiro
Investigador Júnior
Susana Maria Henriques Olhero
Professor AuxiliarProjectos
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work is funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT - Portuguese Foundation for Science and Technology under the 2BBone project (PTDC/CTMCER/29940/2017). The project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC) is also acknowledged. P. M. C. Torres and S M Olhero acknowledge FCT for CEECIND/ 01891/2017 and CEECIND/03393/2017 contracts, respectively. A. Baiao thanks FCT for the financial support (Grant 2021 05027. BD). The authors would also like to thank Dr. Isabel Sousa from CICECO-Aveiro Institute of Materials for the generous help with the analysis and discussion of the results.