Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production


The inadequate supply of oxygen to biomass is a critical factor to the productivity of most aerobic submerged fermentations. This happens because oxygen is sparingly soluble in the aqueous media. The use of a second liquid phase of perfluorocarbon (PFC), an oxygen-carrying compound, in the culture medium can increase the availability of oxygen to the microorganisms. The effect of perfluorodecalin on Yarrowia lipolytica cultures was investigated in shake-flask cultures. It was found that the specific growth rate of Y. lipolytica, a strictly aerobic yeast, increases with increasing PFC concentration. Extracellular lipase production was increased with 20% (v/v) of PFC and agitation of 250 rev/min. It was shown that the PFC presence benefitted lipase production and not just its secretion to the extracellular medium.




Biotechnology & Applied Microbiology


Amaral, PFF; de Almeida, APR; Peixoto, T; Rocha-Leao, MHM; Coutinho, JAP; Coelho, MAZ

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