resumo
Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell Culture assays using MG63 osteoblast-like and human bone marrow, cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) Substrates for NCD coatings based on a previous demonstration Of the Superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue Culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. (C) 2007 Wiley Periodicals, Inc.
palavras-chave
ENHANCED OSTEOBLAST ADHESION; VAPOR-DEPOSITED DIAMOND; COATED SILICON-NITRIDE; NANOPHASE CERAMICS; COATINGS; DIFFERENTIATION; PROLIFERATION; COMPOSITES; CARBON; FILMS
categoria
Engineering; Materials Science
autores
Amaral, M; Dias, AG; Gomes, PS; Lopes, MA; Silva, RF; Santos, JD; Fernandes, MH
nossos autores
Rui Ramos Ferreira e Silva
Professor associadoagradecimentos
Contract grant sponsor: FCT; contract grant numbers: SFRH/BD/9272/2002, POCI/CTM/45423/2002