resumo
The detailed knowledge of mammalian cell metabolism and its adjustments to different cell properties and perturbations, such as disease and drug exposure, is of enormous value in the deeper understanding of pathological processes and drug mechanisms, as well as in the development of new and improved methods for diagnosis, follow-up of disease progression and treatment response. This review covers recent developments in the use of NMR-based metabonomics to characterize cellular metabolomes and interpret them in terms of metabolic changes taking place in a wide range of situations. The analytical methodology available is briefly presented and the applications developed so far are reviewed. These include differences in cell properties (e.g., drug resistance, cell cycle stage, specific growth conditions and genetic characteristics) and changes induced in response to different perturbations (e.g., disease, drug exposure and irradiation).
palavras-chave
MAGNETIC-RESONANCE-SPECTROSCOPY; CHOLINE PHOSPHOLIPID-METABOLISM; IN-VITRO H-1-NMR; HIGH-RESOLUTION; INDUCED APOPTOSIS; MOBILE LIPIDS; CANCER-CELLS; PHARMACODYNAMIC MARKERS; GLUCOSE-METABOLISM; CARCINOMA-CELLS
categoria
Biochemistry & Molecular Biology; Chemistry
autores
Duarte, IF; Lamego, I; Rocha, C; Gil, AM
nossos autores
Ana Maria Pissarra Coelho Gil
Professor Associado com Agregação
Iola Melissa Fernandes Duarte
Investigador Principalagradecimentos
Funding is acknowledged from Fundacao para a Ciencia e Tecnologia. Portugal, in the form of research projects FCT/PTDC/QUI/68017/2006 and FCT/PTDC/SAU-BEB/66896/2006. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.