resumo
Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids). In this work, the supercritical fluid extraction (SFE) of Eucalyptus globulus deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100-200 bar), co-solvent (ethanol) content (0, 5 and 8% wt), and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 degrees C, the introduction of 8% (wt) of ethanol greatly improves the yield of triterpenoids more than threefold.
palavras-chave
CHROMATOGRAPHY-MASS SPECTROMETRY; BIOACTIVE PHENOLIC SUBSTANCES; LIPOPHILIC EXTRACTIVES; CHEMICAL-COMPOSITION; GAS-CHROMATOGRAPHY; CARBON-DIOXIDE; OUTER BARKS; PHYTOSTEROLS; FUTURE; BIOREFINERIES
categoria
Biochemistry & Molecular Biology; Chemistry
autores
Domingues, RMA; Oliveira, ELG; Freire, CSR; Couto, RM; Simoes, PC; Neto, CP; Silvestre, AJD; Silva, CM
nossos autores
Armando Jorge Domingues Silvestre
Professor Catedrático
Carlos Manuel Silva
Professor associado
Carlos Pascoal Neto
Diretor do Raiz
Carmen Sofia da Rocha Freire Barros
Investigador Coordenador
agradecimentos
We acknowledge the 7th Framework Programme FP7/2007-2013 for funding project AFORE: Forest Biorefineries: Added-value from chemicals and polymers by new integrated separation, fractionation and upgrading technologies (CP-IP 228589-2), to the BIIPP project (QREN 11551) for awarding a research grant to Rui Domingues, and to CICECO (Pest-C/CTM/LA0011/2011).