Ana Maria Rebelo Barreto Xavier

Microbial Technology. Biotechnological processes for production and application of enzymes and other metabolites. Kinetics of different types of microbial cultures in different types of bioreactors. Renewable resources and biorefineries. Enzymes and biocatalysis.

Second generation bioethanol production – Focus on pulp and paper industry wastes as feedstock for Bioenergy. Different lignocelulosic biomass pre-treatments, hydrolysis and fermentation.

Ligninolytic enzymatic activity for bleaching processes of pulp and paper industry. Ligninolytic activity for pulp and paper effluent cleaning processes.

Laccase production and induction. Laccase immobilization in nanoparticles and laccase activation with ionic liquids.

Biorefinery - Potentialities of hardwood spent sulfite liquor. Yeasts for bioethanol production, Single Cell Protein and biopolymers production.

Mixed cultures for subproducts valorization.

Lactic acid fermentation from renewable resources. 

Degree (“Licenciatura”) in Aplicated Chemistry - Biotechnology, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal, 23/07/87.
PhD in Biological Engineering, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa (FCT-UNL)under the supervision of Professor Manuel Carrondo. Thesis: “Adsorção de ácido lático e sua produção em fermentador com recirculação celular em membranas de ultrafiltração” (1996). Graduation in Chemistry - Biotechnology, (FCT-UNL).

1993: Assistant (“Assistente Estagiária”), University of Aveiro.

Since 1996: Assistant (“Auxiliar”) Professor, University of Aveiro

Luísa Serafim
Dmytri Evtyugin
Jorge Rocha 
Mariana Amândio

Luísa Serafim
Dmytri Evtyugin
Inês Portugal
Jorge Saraiva

Non-funded Projects
Laccase biorreactor production by Trametes versicolor

Funded Projects
Projecto do Senado da Universidade de Aveiro com colaboração do Departamento de Biologia intitulado “Valorização de produtos agro-industriais de baixo valor” (1997-2000).

Funding: FCT, Fundacão para a Ciência e Tecnologia

POCTI/EQU/48489/2002.

BIOREFINARy – Potencialities of hardwood spent sulfite liquor

Funding  Caima – Indústria de Celulose SA 2007-2008.

- INPACTUS - products § innovative technologies from eucalypt. 2018-2022 UA+ University of Coimbra, The Navigator company. POCI-01-0247-FEDER-021874 ; Funding: PT2020.

 -MULTISMART-Innovative multifunctional nanofibrous membranes for removal of mycotoxins in liquid foods. UA+University of Minho. 2018-202. POCI-01-0145-FEDER-031924; Funding:FCT - 02/SAICT/201

Amândio, M. S. T.; Rocha, J. M. S.; Serafim, L. S.; Xavier, A. M. R. B. Cellulosic bioethanol from industrial Eucalyptus globulus bark residues using kraft pulping as a pretreatment. Energies 2021, 14, 1–19, https://doi.org/10.3390/en14082185    

Rita H. R. Branco, Amândio, M.S.T., Luísa S. Serafim, Ana M.R.B. Xavier. Ethanol Production from Hydrolyzed Kraft Pulp by Mono- and Co-Cultures of Yeasts: The Challenge of C6 and C5 Sugars Consumption. Energies 2020, 13, 744; https://doi.org/10.3390/en13030744

Cristiano C. S. Fortes, Ana L Daniel-da-Silva, Ana P.M. Tavares, Ana M.R.B. Xavier, Optimization of enzyme immobilization on functionalized magnetic nanoparticles for laccase biocatalytic reactions. Chemical Engineering & Processing: Process Intensification, 117,1-8, 2017 https://doi.org/10.1016/j.cep.2017.03.009 

Maria C. Fernandes, Miguel D. Ferro, Ana F.C. Paulino, Joana A.S. Mendes, Janis Gravitis, Dmitry V. Evtuguin, Ana M.R.B. Xavier, Enzymatic saccharification and bioethanol production from Cynara cardunculus pretreated by steam explosion. Bioresource Technology 186, 309–315, 2015 https://doi.org/10.1016/j.biortech.2015.03.037

S.R. Pereira, V.Sànchez i Nogué, C.J R Frazão, L. S. Serafim, M.F.Gorwa-Grauslund, A.M R B Xavier, Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering. Biotechnology for Biofuels 8, 50, 2015. http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0234-y

Joana A.S. Mendes, Ana M.R.B. Xavier, Dmitry V. Evtuguin, Luísa P.C. Lopes. Integrated utilization of grape skins from white grape pomaces. Industrial Crops and Products 49: 286– 291, 2013.

Pereira SR, Nunes DJP, Evtuguin DV, Serafim LS, Xavier AMRB. Advances in ethanol production from hardwood spent sulphite liquors. Process Biochemistry 48: 272–282, 2013.

Ana P.M. Tavares, Juliana A. N. Pereira, Ana M.R.B. Xavier, Effect of Ionic Liquids activation on laccase from Trametes versicolor: enzymatic stability and activity, Engineering in Life Sciences, 12 (5) 1–8, 2012.

Daniel L. A. Fernandes, Carlos M. Silva, Ana M.R.B.Xavier,  Dmitry Evtuguin, Fractionation of Sulphite Spent Liquor Using Ion Exchange Resins for Biochemical Processing, Journal of Biotechnology, 162, 415– 421, 2012.

Jorge A. Saraiva, Ana P. M. Tavares, Ana M. R. B.Xavier, Effect of the Inducers Veratryl Alcohol, Xylidine,and Ligninosulphonates on Activity and Thermal Stability and Inactivation Kinetics of Laccase from Trametes versicolor, Appl Biochem Biotechnol, 167:685–693, 2012.

S. R. Pereira, Š. Ivanuša, D.V. Evtuguin, L. S. Serafim, A. M.R.B. Xavier, Biological treatment of eucalypt spent sulphite liquors: A way to boost the production of second generation bioethanol. Bioresource Technology 103 131–135, 2012.

Xavier, A. M. R. B., Correia, M. F., Pereira, S. R., and Evtyugin, D. V., Second-generation bioethanol from eucalypt sulphite spent liquor. Bioresource Technology, 101: 2755–2761, 2010.

A.P.M. Tavares, M.A.Z. Coelho, M.S.M. Agapito, J.A.P. Coutinho and A.B.M.R. Xavier, Optimization and modelling of laccase production by Trametes versicolor in a Bioreactor using Statistical Experimental Design 2006 Applied Biochemistry and Biotechnology, 134, 233-248.

Gamelas, J.A.F., Tavares, A.P.M., Evtuguin, D.V., Xavier, A.M.R.B. “Oxygen bleaching of kraft pulp with polyoxometalates and laccase applying multi-stage process: comparative study”. Journal of Molecular Catalysis B: Enzymatic, 2005, 33, 57-64.

Tavares, A.P.M., Coelho, M.A.Z., Coutinho, J.A.P., Xavier, A.M.R.B. Laccase improvement in submerged cultivation: induced production and kinetic modeling. Journal of Chemical Technology and Biotechnology, 2005, 80, 669-676.

A.P.M. Tavares, M.A.Z. Coelho, J.A.P. Coutinho, A.M.R.B. Xavier. Laccase improvement in submerged cultivation: induced production and kinetic modelling. Journal of Chemical Technology and Biotechnology, 80 688-696(2005).

J.A.F. Gamelas, A.P.M. Tavares, D.V. Evtuguin, A.M.B. Xavier. Oxygen bleaching of kraft pulp with polyoxometalates and laccase applying multi-stage process: comparative study. Journal of Molecular Catalysis B: Enzymatic, 33 57-64 (2005).

A.M.R.B. Xavier, L.M.D.Gonçalves, J.L.Moreira and M.J. Carrondo,(1995) Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor. Biotechnol.Bioeng. 45: 320-327.

Gonçalves, L.M.D., Xavier, A.M.R.B., Almeida, J.S., Carrondo, M.J.T.,(1991) Concomitant substrate and product inhibition kinectics in lactic acid production. Enz and Microbial Tecnology 13, 314-319.
 

Serafim L.S., Xavier A.M.R.B., Lemos P.C. (2018) Storage of Hydrophobic Polymers in Bacteria. In: Geiger O. (eds) Biogenesis of Fatty Acids, Lipids and Membranes. Handbook of Hydrocarbon and Lipid Microbiology. Springer, Cham.  Online ISBN 978-3-319-43676-0. DOI https://doi.org/10.1007/978-3-319-43676-0_33-1  
 
Ana P M Tavares, Cláudia G Silva and Ana M R B Xavier, Laccase properties, reaction mechanisms and applications: an overview in Laccase, Applications, Investigations and Insights, Edited by Amanda Harris in Biochemistry Research Trends, Nova Publishers, New York, 1st chap, 1-26, 2017. ISBN: 978-1-53610-582-7.
 
Ana P.M. Tavares and Ana M.R.B. Xavier (2014), Application of Oxidative Enzymes in the Treatment of Industrial Effluents, chapter 7 in Biotechnology Vol. 11:  Biodegradation and Biorremediation, 193–214. Studium Press LLC, Houston, TX, USA. ISBN: 1-62699-026-1.
 
Ana P.M. Tavares, Ana M.R.B. Xavier and Dmitry Evtuguin (2014), Biotechnology Applications in Pulp and Paper Industry, chapter 22 in Biotechnology Vol.12: Bioprocess Engineering, 561–581. Studium Press LLC, Houston, TX, USA. ISBN: 1-62699-027-1.
 
Daniel L. A. Fernandes, Susana R. Pereira, Luísa S. Serafim, Dmitry Evtuguin and Ana M.R.B.Xavier (2012) Second Generation Bioethanol from Lignocellulosics: Processing of Hardwood Sulphite Spent Liquor, In Bioethanol, Marco A. P. Lima (Ed.), InTech, . InTech, Rijeka, Croatia pp. 123-152. ISBN 978-953-51-0008-9.

Undergraduate courses: Food Biotechnology, Microbial Biotechnology, Biocatalysts, Unit Operations in Food Processing, Mass and Energetic Balances and Mass Transfer, General Chemical Engineering Chemistry and Biochemistry Pratical classes.

MSc Thesis supervision
“Estudo da actividade lenhinolítica do Trametes versicolor” with co-orientation – 2000
“Estudo da biodegradação da lenhina pelo fungo Trametes versicolor“ with co-orientation – 2001
"Tratamento de efluente de produção de pasta Kraft por Trametes versicolor" with co-orientation - 2007
“Produção de bioetanol a partir de licor de cozimento ao sulfito ácido” with co-orientation, 2008, Theses premiated with first prize of ChemPor Jovem 2008
“Produção de proteína microbiana a partir de licor de cozimento ao sulfito ácido” with co-orientation - 2008
“Optimização de um fermentador de leito fixo para desacidificação biológica de mostos e vinhos" in PROENOL with co-orientation, 2008
“Projecto de optimização do tratamento biológico, com o sistema BioAmp GT, em águas residuais”– in PROQUICHEME, with co-orientation, 2008
“Fermentação contínua de mosto com leveduras imobilizadas em alginato" in PROENOL with co-orientation - 2009
"Tratamento biológico de efluente de produção kraft por Phlebia rufa", with co-orientation - 2009
"Produção, isolamento e caracterização química do exopolissacarídeo do fungo da prodridão branca da madeira Tramentes versicolor", with co-orientation - 2009
"Estudo da estabilidade térmica e bárica da levedura Pichia stipitis", with co-orientation - 2009"
"Bioproccessing of Spent Sulphite Liquor for Bioethanol and Single Cell Protein production", with co-orientation -2010
"Biorefinery: valorisation of pulp and paper industry side-products for bioplastics production", with co-orientation -2010
"Estudo da estabilidade, actividade e cinética enzimática da lacase em líquidos iónicos", with co-orientation - 2010
"Cinética de Produção de Etanol por Pichia stipitis" -", with co-orientation - 2011
“Estudo e optimização do funcionamento de uma ETAR industrial” –with co-orientation - 2012
“Produção de espumante pelo Método Charmat com leveduras imobilizadas” -with co-orientation - 2012
"Produção de bioetanol a partir de SSLs: S. stipitis vs S. cerevisae" -with co-orientation - 2012

“Caraterização de Leveduras Imobilizadas por Citometria de Fluxo”-with co-orientation - 2013

 2 PhD theses supervised
“Produção de lacase para potencial aplicação como oxidante na indústria papeleira” with co-orientation –2006, Theses premiated with an honour mention by 2006-CUF Premium

“Second generation bioethanol production from a sub-product of pulping industry” with co-orientation 2013.