abstract
Bacterial cellulose (BC) has attracted attention as a new functional material due to its excellent mechanical strength, tridimensional nanostructure, high purity, and increased water absorption, compared to plant cellulose. In this work, commercial laccase was immobilized on BC and the influence of enzyme concentration, contact time, and pH was optimized toward the recovery activity of immobilized laccase. This optimization was carried out using a 3(3) experimental design and response surface methodology. Enzyme concentration played a critical role in laccase immobilization. Under optimized conditions (0.15 L L-1 of enzyme concentration, 4.8 h of contact time, pH 5.4), the predicted and experimental response were equal to 47.88 and 49.30%, respectively. The thermal stability of the immobilized laccase was found to increase notably at 60 and 70 degrees C presenting stabilization factor equal to 1.79 and 2.11, respectively. The immobilized laccase showed high operational stability, since it retained 86% of its initial activity after seven consecutive biocatalytic cycles of reaction with 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid). Kinetic studies showed that the values of Michaelis-Menten constant and maximum reaction rate decreased upon immobilization (9.9- and 1.6-fold, respectively). Globally, the use of immobilized laccase on BC offers an interesting tool for industrial biocatalytic applications.
keywords
REACTIVE TEXTILE DYES; PYCNOPORUS-SANGUINEUS LACCASE; GREEN COCONUT FIBER; COMMERCIAL LACCASE; GLUCONACETOBACTER-SACCHARI; ENZYME IMMOBILIZATION; EFFLUENT TREATMENT; DECOLOURIZATION; DEGRADATION; DERIVATIVES
subject category
Biotechnology & Applied Microbiology
authors
Frazao, CJR; Silva, NHC; Freire, CSR; Silvestre, AJD; Xavier, AMRB; Tavares, APM
our authors
Ana Maria Rebelo Barreto Xavier
Assistant Professor
Ana Paula Mora Tavares
Assistant Researcher
Armando Jorge Domingues Silvestre
Full professor
Carmen Sofia da Rocha Freire Barros
Coordinating Researcher
Claudio José Remédios Frazão
MSc Student
Nuno Helder da Cruz Simões Silva
ResearcherGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
acknowledgements
This work was funded by FEDER funds through COMPETE (Programa Operacional Factores de Competitividade) and national funds through FCT (Fundacao para a Ciencia e Tecnologia) under project PEst-C/CTM/LA/0011/2013 and PEst-C/EQB/LA0020/2013. A. P MT. and C. S. R. F. acknowledge the financial support from FCT (Programs