Study of the Alkyl Chain Length on Laccase Stability and Enzymatic Kinetic with Imidazolium Ionic Liquids
authors Rodriguez, O; Cristovao, RO; Tavares, APM; Macedo, EA
nationality International
journal APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
author keywords Ionic liquids; Laccase; Stability; Activity; Kinetic parameters
keywords REACTIVE TEXTILE DYES; CERRENA-UNICOLOR; FUNGAL LACCASES; ORGANIC MEDIA; OXIDATION; HYDROLYSIS; LIPASE; WATER; DECOLORIZATION; DEGRADATION
abstract The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C(4)mim][Cl], 1-octyl-3-methyl imidazolium chloride [C(8)mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C(10)mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C(4)mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C(10)mim][Cl] > [C(8)mim][Cl] > [C(4)mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis-Menten parameters. Among the ILs, [C(4)mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.
publisher HUMANA PRESS INC
issn 0273-2289
year published 2011
volume 164
issue 4
beginning page 524
ending page 533
digital object identifier (doi) 10.1007/s12010-010-9154-2
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000290227000011
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