Studies of laccase from Trametes versicolor in aqueous solutions of several methylimidazolium ionic liquids
authors Dominguez, A; Rodriguez, O; Tavares, APM; Macedo, EA; Longo, MA; Sanroman, MA
nationality International
journal BIORESOURCE TECHNOLOGY
author keywords Trametes versicolor; Laccase; Ionic liquids; Stability; Kinetic behavior
keywords CATALYTIC-ACTIVITY; THERMAL-STABILITY; FUNGAL LACCASES; ENZYME-ACTIVITY; OXIDATION; BIOCATALYSIS; PURIFICATION; PEROXIDASE; REACTIVITY; BIOSENSORS
abstract Stability and kinetic behavior of laccase from Trametes versicolor in the presence of several ionic liquids from the methylimidazolium family have been investigated. In general laccase stability diminished as the size of the alkylic substitute in the methylimidazolium ring increased. Higher concentrations of ionic liquids caused more destabilization than lower ones. Thus, low concentrations of [C(2)mim(+)][EtSO4-] allowed maintaining enzymatic stability. [C(4)mim(+)][Cl-] appeared to have a stabilizing effect on laccase, as little activity decay was observed within three weeks. Kinetic studies indicated that both [C(2)mim(+)][EtSO4-] and [C(4)mim(+)][Cl-] inhibited laccase activity, although 10-fold more [C(2)mim(+)][EtSO4-] than [C(4)mim(+)][Cl-] was required to cause the same degree of inhibition. A kinetic model was developed to represent the experimental data. (C) 2011 Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 0960-8524
year published 2011
volume 102
issue 16
beginning page 7494
ending page 7499
digital object identifier (doi) 10.1016/j.biortech.2011.05.063
web of science category Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
subject category Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels
unique article identifier WOS:000293204700017
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