Studies of laccase from Trametes versicolor in aqueous solutions of several methylimidazolium ionic liquids

abstract

Stability and kinetic behavior of laccase from Trametes versicolor in the presence of several ionic liquids from the methylimidazolium family have been investigated. In general laccase stability diminished as the size of the alkylic substitute in the methylimidazolium ring increased. Higher concentrations of ionic liquids caused more destabilization than lower ones. Thus, low concentrations of [C(2)mim(+)][EtSO4-] allowed maintaining enzymatic stability. [C(4)mim(+)][Cl-] appeared to have a stabilizing effect on laccase, as little activity decay was observed within three weeks. Kinetic studies indicated that both [C(2)mim(+)][EtSO4-] and [C(4)mim(+)][Cl-] inhibited laccase activity, although 10-fold more [C(2)mim(+)][EtSO4-] than [C(4)mim(+)][Cl-] was required to cause the same degree of inhibition. A kinetic model was developed to represent the experimental data. (C) 2011 Elsevier Ltd. All rights reserved.

keywords

CATALYTIC-ACTIVITY; THERMAL-STABILITY; FUNGAL LACCASES; ENZYME-ACTIVITY; OXIDATION; BIOCATALYSIS; PURIFICATION; PEROXIDASE; REACTIVITY; BIOSENSORS

subject category

Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels

authors

Dominguez, A; Rodriguez, O; Tavares, APM; Macedo, EA; Longo, MA; Sanroman, MA

our authors

Share this project:

Related Publications

We use cookies for marketing activities and to offer you a better experience. By clicking “Accept Cookies” you agree with our cookie policy. Read about how we use cookies by clicking "Privacy and Cookie Policy".