Ildefonso Marin Montesinos

Junior Researcher

Contact

Department

  • Chemistry
  • 15.3.20

Research

Links

Scientic Interests

Methodology

  • Methodology in solid-state NMR and Magic-Angle Spinning Dynamic Nuclear Polarization (MAS-DNP) techniques
  • Developing of protocols  and novel radicals for MAS-DNP
  • Dipolar recoupling techniques by symmetry-based pulse sequences
  • Other characterization techniques : Neutron scattering and refletometry, QCMd, AFM, ellipsometry.
  • Sample preparation of lipidic samples: Langmuir-Blodgett film formation and liposome formation techniques

Materials

  • Characterization of materials for gas retention (CO2, SF6) by MAS-DNP solid-state NMR
  • NMR and MAS-DNP characterization of porous materials for application in catalysis
  • Use of novel materials from different waste for gas adsorption
  • Conversion/adsorption of CO2 by amyloidal protein ensembles

Biology

  • Protein-Ligand interactions
  • Interaction site structure in protein-ligand binding
  • Characterization and study of membrane associated processes and biomolecules
  • Antimicrobial peptides
  • MAS-DNP and NMR characterization of biological systems
  • Structural study of the interaction interfaces of adaptor proteins involved in clathrin-mediated endocytosis (CME)
  • CME and its implications in degenerative diseases

Short CV

I obtained my BSc in Chemistry at the Universidad de Sevilla in 2001. By October 2003, I started a PhD programme funded by an EPSRC grant in the subject of Solid-State NMR (ssNMR) in the well-known group of Prof. Malcolm H. Levitt at the University of Southampton (UK). During my PhD, I acquired a solid theoretical and experimental knowledge of the NMR technique. As main outcome, I developed new methodology to improve homonuclear recoupling pulse sequences for the measurement of atomic distance, and its application to protein studies.
In November 2007, I joined the group of Prof. Ulrich Günther at University of Birmingham, where I entered in contact for the first time with the Dynamic Nuclear Polarization (DNP) technique, which is revolutionizing NMR, improving by far the sensitivity limits of the technique with an important impact in areas like surface chemistry. These works were performed in close collaboration with the industrial partner Oxford Instruments Biotools, using the HypeSense equipment. I worked in new fast NMR methodology for its application in dissolution-DNP, the study of the polarization transfer mechanisms in DNP and the development of hardware for dissolution-DNP.
By the end of 2010, I joined the Biomolecular NMR Laboratory of Prof. Miquel Pons at the IRB-Barcelona and the Universitat de Barcelona, after obtaining a “Beatriu de Pinos” and a “Juan de la Cierva” grants. The main research lines carried out were the characterization of paramagnetic supramolecular oligomers formed by trityl radicals, design and testing of new polarizing agents for DNP, the hyperpolarization of quadrupolar nuclei, like 14N, and the study of protein-ligand interactions by DNP-NMR.
In 2015, I earned a Labex ARCANE Postdoctoral Fellowship  to work in the laboratory of Dr. Gaël de Paëpe and Dr. Sabine Hediger at the CEA-Grenoble and Université Grenoble-Alpes in Grenoble (France). The main research line was the study of carbohydrate-protein interaction by MagicAngle-Spinning DNP (MAS-DNP) solid-state NMR in Lectins and application of MAS-DNP to the study of biomolecular problems. Additional projects involve the design and testing of new polarizing agents for MAS-DNP and its application to mesoporous materials, the mechanistic study of the polarization transfer process between electrons and nuclei, and the investigation of the influence of O2 in MAS-DNP experiments.
Since January 2019, I have joined the CICECO institute of the Universidade de Aveiro to work in study of materials for the trapping of CO2 by ssNMR and MAS-DNP in collaboration with Dr. Mariana Sardo and Dr. Luis Mafra. Additionally, I will develop my own research line in biomolecular MAS-DNP with a project to selectively enlightening of the interaction interfaces of clathrin adaptor proteins involved in clathrin-mediated endocytosis.

Research group

Team Specko: http://specko.web.ua.pt

 

Ongoing Supervisions

Francisco José Sequeira Vicente

Research Fellowship

Márcio Miguel da Silva Soares

PhD Student

Rita Maria Bolota Velho Pires da Fonseca

PhD Student

Vítor J. Martins

PhD Student

Publications

Assessing CO2 Capture in Porous Sorbents via Solid-State NMR-Assisted Adsorption Techniques

Marina Ilkaeva, Ricardo Vieira, João Pereira, Mariana Sardo, Ildefonso Marín Montesinos and Luís Mafra
2023, Journal of the Americam Chemical Society.

Understanding CO2 adsorption mechanisms in porous adsorbents: A solid-state NMR survey

Daniel Pereira, Rita Fonseca, Ildefonso Marin-Montesinos, Mariana Sardo, Luís Mafra
2023, Current Opinion in Colloid & Interface Science, 60, Special issue NMR 2022.

Engineering Phosphatidylinositol-4,5-bisphosphate model membranes enriched 1 in endocytic cargo: a neutron reflectometry, AFM and QCM-D structural study

Daniel Pereira, Andreas Santamaria, Nisha Pawar, Javier Carrascosa-Tejedor, Mariana Sardo, Luis Mafra, Eduardo Guzman, David J. Owen, Nathan R. Zaccai, Armando Maestro, Ildefonso Marin-Montesinos
2023, Colloids and Surfaces B: Biointerfaces, 227.

Contribution of non-ionic interactions on bile salt sequestration by chitooligosaccharides: Potential hypocholesterolemic activity

Filipe Coreta-Gomes, Inês M.V. Silva, Claudia Nunes, Ildefonso Marin-Montesinos, Dmitry Evtuguin, Carlos F.G.C. Geraldes, Maria João Moreno, Manuel A. Coimbra
2023, Journal of Colloid and Interface Science, 646 .

Exploring Molecular Dynamics of Adsorbed CO2 Species in Amine- Modified Porous Silica by Solid-State NMR Relaxation

Fonseca, R; Vieira, R; Sardo, M; Marin-Montesinos, I; Mafra, L
2022, JOURNAL OF PHYSICAL CHEMISTRY C, 126, 30, 12582-12591.

Targeted DNP for biomolecular solid-state NMR

Gauto, D; Dakhlaoui, O; Marin-Montesinos, I; Hediger, S; De Paepe, G
2021, CHEMICAL SCIENCE, 12, 18, 6223-6237.
ISBN: 2041-6539

Hidden CO2 in Amine-Modified Porous Silicas Enables Full Quantitative NMR Identification of Physi- and Chemisorbed CO2 Species

Vieira, R; Marin-Montesinos, I; Pereira, J; Fonseca, R; Ilkaeva, M; Sardo, M; Mafra, L
2021, JOURNAL OF PHYSICAL CHEMISTRY C, 125, 27, 14797-14806.
ISBN: 1932-7455

Selective High-resolution DNP-enhanced NMR of Biomolecular Binding Sites

Ildefonso Marin-Montesinos, David Goyard, Emilie Gillon, Olivier Renaudet, Anne Imberty,Sabine Hediger and Gaël De Paëpe
2019, Chemical Science, 10, 3366.

Detecting acetylated aminoacids in blood serum using hyperpolarized C-13-H-1-2D-NMR

Katsikis, S; Marin-Montesinos, I; Ludwig, C; Gunther, UL
2019, JOURNAL OF MAGNETIC RESONANCE, 305, 175-179.
ISBN: 1096-0856

Computationally Assisted Design of Polarizing Agents for Dynamic Nuclear Polarization Enhanced NMR: The AsymPol Family

F. Mentink-Vigier*, I. Marín-Montesinos*, A. P. Jagtap, J. van Tol, S. Hediger, D. Lee, S. Th. Sigurdsson, and G. De Paëpe
2018, Journal of the American Chemical Society, 140, 11013 -11019.

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