Ildefonso Marin Montesinos

Junior Researcher

Scientic Interests

Methodology

  • Methodology in solid-state NMR and Magic-Angle Spinning Dynamic Nuclear Polarization (MAS-DNP) techniques
  • Developing of protocols  and novel radicals for MAS-DNP
  • Dipolar recoupling techniques by symmetry-based pulse sequences
  • Other characterization techniques: Neutron scattering and reflectometry, QCMd, AFM, ellipsometry, XRD, FT-IR, Langmuir-Blodgett isotherms, volumetric adsorption analysis, thermogravimetric analysis.
  • Sample preparation of lipidic samples: Langmuir-Blodgett film formation and liposome formation techniques

Materials

  • Characterization of materials for gas capture applications
  • Characterization of materials for application in catalysis
  • Novel materials from different waste for gas adsorption
  • Novel Materials for CO2 adsorption/conversion

Biology

  • Protein-Ligand interactions
  • Interaction site structure in protein-ligand binding
  • Characterization and study of membrane associated processes and biomolecules
  • Antimicrobial peptides
  • MAS-DNP and NMR characterization of biological systems
  • Structural study of the interaction interfaces of adaptor proteins involved in clathrin-mediated endocytosis (CME)
  • CME and its implications in degenerative diseases

Short CV

ACADEMIC BACKGROUND

I obtained my BSc. in Chemistry at the Universidad de Sevilla in 2001. By October 2003, I started a PhD funded by an EPSRC Grant in the development of recoupling methods in Solid-State NMR for biomolecules in the group of Malcolm H. Levitt (University of Southampton). RESEARCH CAREER In November 2007, I joined the group of Ulrich Günther (University of Birmingham) as post-doc, where I entered in contact for the first time with the Dynamic Nuclear Polarization (DNP) technique. In 2010, I obtained the Beatriu de Pinós and Juan de La Cierva grants to work at the Biomolecular NMR Laboratory with Miquel Pons (IRB-Barcelona and the Universitat de Barcelona). In this lab, I started building my network of collaborators, further strengthening my knowledge of DNP and protein-ligand interactions. From 2015-2018, I was awarded a Labex ARCANE Postdoctoral Fellowship in the laboratory of Gaël de Paëpe and Sabine Hediger (CEA-Grenoble and Université Grenoble-Alpes). This position allowed me to further improve my knowledge of DNP by working in the study of carbohydrate-protein interaction by Magic-Angle-Spinning DNP (MAS-DNP) solid-state NMR in lectins. In 2019, I joined CICECO-Aveiro Institute of Materials as a researcher to work in the team of Luis Mafra using NMR and MAS-DNP for the characterization of porous materials and biomaterials with multiple applications such as gas separation/capture and catalysis. Additionally, I am establishing my own independent research line on structural biology with two main projects (see below).

CURRENT RESEARCH LINES

a) Biochemistry/Biology:

  1. Study interaction interfaces in clathrin-mediated endocytosis using complementary characterization techniques (solid-state NMR, neutron reflectometry, QCMD)
  2. Study the interaction of antimicrobial peptides with mimetic membranes
  3. Brewer spent yeast glucan structural characterization and its role on receptor recognition

b) Porous materials for CO2 capture:

  1. Amine-modified silicas
  2. Valorization of industrial bioresidues
  3. Covalent Organic Frameworks
  4. Periodic mesoporous organo-silicas
  5. Polysaccharide-derived solid CO2
  6. Lithium silicate sorbents. Different studies are performed for the characterization of these materials for CO2 capture. Amongst others, adsorption isotherms, NMR/DNP experiments for speciation and quantification, degradation under different conditions of humidity and temperature and recyclability assays.

c) NMR/DNP methodological developments:

  1. 3D-printing of NMR probe components.
  2. New radicals and sample formulation for MAS-DNP in porous materials and lipid membranes.

Research group

Team Specko: http://specko.web.ua.pt

 

Ongoing Supervisions

Publications

Covalent connectivity of glycogen in brewer's spent yeast cell walls revealed by enzymatic approaches and dynamic nuclear polarization NMR

Rita Bastos, Ildefonso Marín-Montesinos, Sónia S. Ferreira, Frédéric Mentink-Vigier, Mariana Sardo, Luís Mafra, Manuel A. Coimbra, Elisabete Coelho
2024, Carbohydrate Polymers, 324.

Assessing CO2 Capture in Porous Sorbents via Solid-State NMR-Assisted Adsorption Techniques

Marina Ilkaeva, Ricardo Vieira, João Pereira, Mariana Sardo, Ildefonso Marín Montesinos and Luís Mafra
2023, Journal of the Americam Chemical Society.

Understanding CO2 adsorption mechanisms in porous adsorbents: A solid-state NMR survey

Daniel Pereira, Rita Fonseca, Ildefonso Marin-Montesinos, Mariana Sardo, Luís Mafra
2023, Current Opinion in Colloid & Interface Science, 60, Special issue NMR 2022.

Engineering Phosphatidylinositol-4,5-bisphosphate model membranes enriched 1 in endocytic cargo: a neutron reflectometry, AFM and QCM-D structural study

Daniel Pereira, Andreas Santamaria, Nisha Pawar, Javier Carrascosa-Tejedor, Mariana Sardo, Luis Mafra, Eduardo Guzman, David J. Owen, Nathan R. Zaccai, Armando Maestro, Ildefonso Marin-Montesinos
2023, Colloids and Surfaces B: Biointerfaces, 227.

Contribution of non-ionic interactions on bile salt sequestration by chitooligosaccharides: Potential hypocholesterolemic activity

Filipe Coreta-Gomes, Inês M.V. Silva, Claudia Nunes, Ildefonso Marin-Montesinos, Dmitry Evtuguin, Carlos F.G.C. Geraldes, Maria João Moreno, Manuel A. Coimbra
2023, Journal of Colloid and Interface Science, 646 .

One-Shot Resin 3D-Printed Stators for Low-Cost Fabrication of Magic-Angle Spinning NMR Probeheads

Daniel Pereira, Mariana Sardo, Ildefonso Marín-Montesinos, an Luís Mafra
2023, Anal. Chem., 95, 27.

Exploring Molecular Dynamics of Adsorbed CO2 Species in Amine- Modified Porous Silica by Solid-State NMR Relaxation

Fonseca, R; Vieira, R; Sardo, M; Marin-Montesinos, I; Mafra, L
2022, JOURNAL OF PHYSICAL CHEMISTRY C, 126, 30, 12582-12591.

Targeted DNP for biomolecular solid-state NMR

Gauto, D; Dakhlaoui, O; Marin-Montesinos, I; Hediger, S; De Paepe, G
2021, CHEMICAL SCIENCE, 12, 18, 6223-6237.
ISBN: 2041-6539

Hidden CO2 in Amine-Modified Porous Silicas Enables Full Quantitative NMR Identification of Physi- and Chemisorbed CO2 Species

Vieira, R*; Marin-Montesinos, I*; Pereira, J; Fonseca, R; Ilkaeva, M; Sardo, M; Mafra, L
2021, JOURNAL OF PHYSICAL CHEMISTRY C, 125, 27, 14797-14806.
ISBN: 1932-7455

Selective High-resolution DNP-enhanced NMR of Biomolecular Binding Sites

Ildefonso Marin-Montesinos, David Goyard, Emilie Gillon, Olivier Renaudet, Anne Imberty,Sabine Hediger and Gaël De Paëpe
2019, Chemical Science, 10, 3366.

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