Remodeling of liver phospholipidomic profile in streptozotocin-induced diabetic rats
authors Simoes, C; Domingues, P; Ferreira, R; Amado, F; Duarte, JA; Vitorino, R; Neuparth, MJ; Nunes, C; Rocha, C; Duarte, I; Domingues, MR
nationality International
journal ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
author keywords Phospholipid classes; Fatty acid remodeling; Type 1 diabetes mellitus; Mass spectrometry; Nuclear magnetic resonance; Liquid chromatography
keywords FATTY-ACID COMPOSITION; PHOSPHATIDYLCHOLINE; MELLITUS; DISEASE; TYPE-1; COMPLICATIONS; LIPIDS; MODEL
abstract Lipid homeostasis in liver is known to be altered with diabetes mellitus, ultimately leading to liver damage and related complications. The present work aimed to evaluate changes in the liver phospholipid profile after 4 months of uncontrolled hyperglycemia. Twenty Wistar rats were divided into two groups: control and streptozotocin-treated (T1DM). After 4 months, animals were sacrificed and morphological characterization of liver was performed and related with serum markers of hepatic damage. Lipid extracts were obtained from liver and phospholipid (PL) classes were quantified. Lipid molecular species were determined by LC-MS and LC-MS/MS, and fatty acids by GC-MS. Concomitantly with signs of hepatic damage we found variations in the relative amount of phospholipid classes in T1DM, characterized by a decrease in PLs with choline head group, and by an increase in the relative content of other PL classes. A remodeling in PL fatty acyl chains was observed in T1DM liver, with a similar pattern to all the PL classes, and consisting in the reduction of 16:0 and an increase of 18:0 and 18:2 acyl chains. The observed changes in T1DM lipid profile may contribute to the altered membrane properties underlying hepatic damage, worsening the metabolic alterations that characterize T1DM. (c) 2013 Elsevier Inc. All rights reserved.
publisher ELSEVIER SCIENCE INC
issn 0003-9861
year published 2013
volume 538
issue 2
beginning page 95
ending page 102
digital object identifier (doi) 10.1016/j.abb.2013.07.029
web of science category Biochemistry & Molecular Biology; Biophysics
subject category Biochemistry & Molecular Biology; Biophysics
unique article identifier WOS:000325737400005
  ciceco authors
  impact metrics
journal analysis (jcr 2019):
journal impact factor 3.391
5 year journal impact factor 3.48
category normalized journal impact factor percentile 63.089
dimensions (citation analysis):
altmetrics (social interaction):



 


Sponsors

1suponsers_list_ciceco.jpg