abstract
Sterilization of biomaterials by gamma irradiation should not change its structure, since this may affect also its bioactivity. In this work, dextrin and periodate-oxidized dextrin (ODEX), both irradiated and non-irradiated, were hydrolysed by alpha-amylase and the obtained oligosaccharides were fractionated by ligand-exchange/size exclusion chromatography and analyzed by matrix-assisted laser desorption/ionization (MALDI-MS) and electrospray ionization (ESI-MSn) mass spectrometry. This allowed the identification of structures formed during partial periodate oxidation, which displayed aldehyde groups in different positions of dextrin backbone, according to the type of residue which was oxidized. Importantly, gamma irradiation did not structurally change either dextrin or ODEX, showing that it can be used as suitable terminal sterilization method of these materials for biomedical applications. To our best knowledge, this is the first report using MS-based techniques to evaluate the effects of partial periodate oxidation and of gamma irradiation on polysaccharides and, in particular, on dextrin and its oxidized form.
keywords
SIZE-EXCLUSION CHROMATOGRAPHY; RADIATION-INDUCED DEGRADATION; SODIUM ALGINATE; OXIDIZED DERIVATIVES; VISCOSITY DETECTORS; HYDROXYL RADICALS; AQUEOUS SOLUTIONS; LIGHT-SCATTERING; CHAIN STIFFNESS; STARCH
subject category
Polymer Science
authors
Pereira, I; Simoes, J; Evtyugin, DV; Rouif, S; Coimbra, MA; Domingues, MRM; Gama, M
our authors
Dmitry Dmitrievitch Evtyugin
PhD Student
Dmitry Evtyugin
Associate Professor with Aggregationacknowledgements
Isabel Pereira was supported by the grant SFRH/BD/90066/2012 from Fundacao para a Ciencia e a Tecnologia (FCT), Portugal. The authors acknowledge the funding from QREN ("Quadro de Referencia Estrategica Nacional"), ADI ("Agenda de Inovacao") through the project Norte-07-0202-FEDER-038853, and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte.; Also thanks to FCT/MEC Portugal for the financial support to the QOPNA research Unit (FCT UID/QUI/00062/2013), through national founds and where applicable co-financed by the FEDER, within the PT2020 Partnership Agreement, and RNEM (REDE/1504/REM/2005 that concerns the Portuguese Mass Spectrometry Network). Thanks, are also due to FCT for the grant of Joana Sinfoes (SFRH/BPD/90447/2012).