abstract
Herein, thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recovery and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes.
keywords
AMMONIUM-BASED ZWITTERIONS; IONIC LIQUIDS; POLYETHYLENE-GLYCOL; 2-PHASE SYSTEMS; ORGANIC-SOLVENTS; PHASE; EXTRACTION; PROTEINS; BEHAVIOR; LACCASE
subject category
Chemistry; Science & Technology - Other Topics
authors
Ferreira, AM; Passos, H; Okafuji, A; Tavares, APM; Ohno, H; Freire, MG; Coutinho, JAP
our authors
Ana Maria da Conceição Ferreira Takahashi
Junior Researcher
Ana Paula Mora Tavares
Assistant Researcher
Helena Isabel Sousa Passos
Junior Researcher
Mara Guadalupe Freire Martins
Coordinating ResearcherGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
Projects
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2013)
Igy Technology: A Purication Platform using Ionic-Liquid-Based Aqueous Biphasic Systems (IGYPURTECH)
Projeto de Investigação Exploratória: Ana Paula Tavares (IF Ana Paula Tavares)
acknowledgements
This work was developed in the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013), financed by national funds through the FCT/MEC and when applicable co-financed by FEDER under the PT2020 Partnership Agreement. The authors also acknowledge FCT for the PhD grants SFRH/BD/85248/2012 and SFRH/BD/92200/2013 of H. P. and A. M. F., respectively. A. M. F. and H. P. thank the financial support of Professor H. Ohno during their stay in his laboratory for collaboration work. A. P. M. Tavares acknowledges FCT for the Investigator Programme (IF/01634/2015). The authors acknowledge financial support from the European Union Framework Programme for Research and Innovation HORIZON 2020, under the TEAMING Grant agreement No. 739572 - The Discoveries CTR. M. G. F. acknowledges the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC Grant 337753.