Trametes versicolor growth and laccase induction with by-products of pulp and paper industry
authors Xavier, AMRB; Tavares, APM; Ferreira, R; Amado, F
nationality International
journal ELECTRONIC JOURNAL OF BIOTECHNOLOGY
author keywords inducers; laccase; pulp and paper by-products; Trametes versicolor
keywords WHITE-ROT FUNGI; KRAFT PULP; PHANEROCHAETE-CHRYSOSPORIUM; LIGNINOLYTIC FUNGI; DEGRADATION; DYES; 1-HYDROXYBENZOTRIAZOLE; BIODECOLORIZATION; PEROXIDASE; EFFLUENT
abstract The cultivation of Trametes versicolor for laccase production and cell growth were strongly dependent on experimental conditions namely physical and chemical parameters as well as nutrient availability and inducer stimulation. Biomass growth was compared for a rich medium and for a defined medium in two different temperatures. The best temperature was 28 degrees C and the maximum specific growth rates were mu(max) = 0.083 h(-1) for the rich medium and mu(max) = 0.043 h(-1) for the defined medium. It was clearly shown that laccase production is not associated with cell growth, indicating that this ligninolytic enzyme must be produced in the defined medium by a secondary metabolism. In order to obtain laccase induction, addition of solid lignin, lignosulphonates, veratryl alcohol, xylidine and ethanol was tested at different concentrations. To optimise laccase activity, the combined effect of inducer addition and simultaneously glucose suppression was studied. The best result for laccase induction (1240 U/L) was obtained with solid lignin, a by-product of pulp and paper industry and the higher laccase activity attained (1583 U/L) was obtained with the combined effect of xylidine addition and glucose suppression.
publisher UNIV CATOLICA DE VALPARAISO
issn 0717-3458
year published 2007
volume 10
issue 3
beginning page 444
ending page 451
digital object identifier (doi) 10.2225/vol10-issue3-fulltext-1
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000252397200012
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journal impact factor 2.894
5 year journal impact factor 2.872
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