|
authors |
Tavares, APM; Coelho, MAZ; Coutinho, JAP; Xavier, AMRB |
|
nationality |
International |
|
journal |
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY |
|
author keywords |
carbon limitation; copper; inducers; laccase; Trametes versicolor; modelling |
|
keywords |
FUNGUS TRAMETES-VERSICOLOR; KRAFT PULP; PLEUROTUS-OSTREATUS; LIGNIN PEROXIDASE; DECOLORIZATION; ENZYME; OXIDATION; GROWTH; DYES; 1-HYDROXYBENZOTRIAZOLE |
|
abstract |
Improvement of laccase production by Trametes versicolor was made by employing different operational strategies. In the cell growth medium, various glucose concentrations were compared for improving laccase production. A clear and significant stimulation of enzyme production under carbon limitation was obtained. Copper, 2,S-xylidine, and a phenolic mixture were also used as laccase inducers. A cooperative effect between the inducers on laccase production was identified. Mixtures of inducers produced higher laccase activities, reaching values of 5500 U dm(-3). Further productivity enhancement can be obtained using the inducers along with the carbon limitation strategy. It is shown that low laccase concentrations are obtained by a primary metabolism of T versicolor, and that phenolic compounds and carbon limitation induce a secondary metabolism, providing higher laccase concentrations. A mathematical model for laccase production based on a direct experimental measure of biomass, along with substrate consumption and enzymatic activity over time is proposed for non-homogeneous fermentations of T versicolor. (c) 2005 Society of Chemical Industry. |
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publisher |
WILEY-BLACKWELL |
|
issn |
0268-2575 |
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year published |
2005 |
|
volume |
80 |
|
issue |
6 |
|
beginning page |
669 |
|
ending page |
676 |
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digital object identifier (doi) |
10.1002/jctb.1246 |
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web of science category |
Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical |
|
subject category |
Biotechnology & Applied Microbiology; Chemistry; Engineering |
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unique article identifier |
WOS:000229441800007
|
