Laccase improvement in submerged cultivation: induced production and kinetic modelling
authors Tavares, APM; Coelho, MAZ; Coutinho, JAP; Xavier, AMRB
nationality International
journal JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
author keywords carbon limitation; copper; inducers; laccase; Trametes versicolor; modelling
keywords FUNGUS TRAMETES-VERSICOLOR; KRAFT PULP; PLEUROTUS-OSTREATUS; LIGNIN PEROXIDASE; DECOLORIZATION; ENZYME; OXIDATION; GROWTH; DYES; 1-HYDROXYBENZOTRIAZOLE
abstract Improvement of laccase production by Trametes versicolor was made by employing different operational strategies. In the cell growth medium, various glucose concentrations were compared for improving laccase production. A clear and significant stimulation of enzyme production under carbon limitation was obtained. Copper, 2,S-xylidine, and a phenolic mixture were also used as laccase inducers. A cooperative effect between the inducers on laccase production was identified. Mixtures of inducers produced higher laccase activities, reaching values of 5500 U dm(-3). Further productivity enhancement can be obtained using the inducers along with the carbon limitation strategy. It is shown that low laccase concentrations are obtained by a primary metabolism of T versicolor, and that phenolic compounds and carbon limitation induce a secondary metabolism, providing higher laccase concentrations. A mathematical model for laccase production based on a direct experimental measure of biomass, along with substrate consumption and enzymatic activity over time is proposed for non-homogeneous fermentations of T versicolor. (c) 2005 Society of Chemical Industry.
publisher WILEY-BLACKWELL
issn 0268-2575
year published 2005
volume 80
issue 6
beginning page 669
ending page 676
digital object identifier (doi) 10.1002/jctb.1246
web of science category Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical
subject category Biotechnology & Applied Microbiology; Chemistry; Engineering
unique article identifier WOS:000229441800007
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journal analysis (jcr 2019):
journal impact factor 2.75
5 year journal impact factor 2.857
category normalized journal impact factor percentile 53.374
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