abstract
Cuttlefish bone (CB) has been explored as biomaterial in the bone tissue-engineering field due to its unique porous structure and capacity of the aragonite mineral to be hydrothermally converted into calcium phosphates (CaPs). In the present study, undoped and ion (Sr2+, Mg2+ and/or Zn2+) doped biphasic calcium phosphate (BCP) scaffolds were prepared by hydrothermal transformation (HT, 200 degrees C, 24 h) of CB. The obtained scaffolds were sintered and then coated with two commercial polymers, poly(epsilon-caprolactone) (PCL) or poly(DL-lactide) (PDLA), and with two synthesized ones, a poly(ester amide) (PEA) or a poly(ester urea) (PEU) in order to improve their compressive strength. The scaffolds were characterized by X-ray diffraction (XRD) coupled with structural Rietveld refinement, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). The results demonstrate that CB could be entirely transformed into BCPs in the presence or absence of doping elements. The initial CB structure was preserved and the polymeric coatings did not jeopardize the interconnected porous structure. Furthermore, the polymeric coatings enhanced the compressive strength of the scaffolds. The in vitro bio-mineralization upon immersing the scaffolds into simulated body fluid (SBF) demonstrated the formation of bone-like apatite surface layers in both uncoated and coated scaffolds. Overall, the produced scaffolds exhibit promising properties for bone tissue engineering applications.
keywords
POLY(ESTER AMIDE)S; HYDROXYAPATITE SCAFFOLD; POROUS HYDROXYAPATITE; MECHANICAL-PROPERTIES; IN-VITRO; TISSUE; CERAMICS; MORPHOLOGY; DEFECTS; PEPTIDE
subject category
Materials Science
authors
Neto, AS; Fonseca, AC; Abrantes, JCC; Coelho, JFJ; Ferreira, JMF
our authors
João Carlos de Castro Abrantes
Professor Adjunto
José Maria da Fonte Ferreira
Retired ProfessorGroups
G3 - Electrochemical Materials, Interfaces and Coatings
G5 - Biomimetic, Biological and Living Materials
acknowledgements
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/MCTES. Ana S. Neto acknowledges to AdvaMTech, the PhD Program on Advanced Materials and Processing for the PhD grant, PD/BD/114132/2015, founded by the Portuguese Foundation for Science and Technology (FCT). The authors are very thankful to Dias de Sousa - Instrumentacao Analitica e Cientifica, S.A., Setubal, Portugal, for doing the mu-CT analysis of our samples.