abstract
The melanoidin from soluble coffee is utilized as a material-independent, multipurpose coating material. Instantaneous complexation of the coffee melanoidin (CM) with ferric ion (Fe3+) leads to surface-adhesive aggregates, inducing sequential film deposition. Various chemical groups of the CM also allow for post-functionalizations of the CM film, including surface-initiated, ring-opening polymerization and bioinspired silicification. In addition, the CM-based coating is applied to single-cell nanoencapsulation with a strategy of biphasic interfacial reactions. The method is highly cytocompatible (viability >98%), and the CM shell is cytoprotective against lytic enzymes. The coated cells inherit the characterictics of the CM, such as post-functionalizability and antioxidant property. Considering that surface-coating technologies with cytocompatible natural polymers have widely been used for engineering bioentities, the CM-based coating strategy would provide an advanced option for biomedical applications.
keywords
SURFACE-INITIATED POLYMERIZATION; RING-OPENING POLYMERIZATION; INDIVIDUAL MAMMALIAN-CELLS; RADICAL POLYMERIZATION; MOLECULAR-WEIGHT; P-DIOXANONE; THIN-FILMS; ENCAPSULATION; BIOSILICIFICATION; POLYSACCHARIDES
subject category
Chemistry; Science & Technology - Other Topics; Materials Science
authors
Kim, JY; Kim, S; Han, S; Han, SY; Passos, CP; Seo, J; Lee, H; Kang, EK; Mano, JF; Coimbra, MA; Park, JH; Choi, IS
our authors
João Mano
Vice Directoracknowledgements
This work was supported by the Nano Material Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2009-0082580), and developed within the scope of the project CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/MCTES. And we would like to thank the UA and FCT/MCT for the financial support for the QOPNA research Unit (FCT UID/QUI/00062/2019) through national founds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement, and to the Portuguese NMR Network. Claudia Passos contract (CEECIND/01873/2017) was supported by FCT.