PLGA nanoparticles loaded with Gallic acid- a constituent of Leea indica against Acanthamoeba triangularis


Acanthamoeba, a genus that contains at least 24 species of free-living protozoa, is ubiquitous in nature. Successful treatment of Acanthamoeba infections is always very difficult and not always effective. More effective drugs must be developed, and medicinal plants may have a pivotal part in the future of drug discovery. Our research focused on investigating the in vitro anti- acanthamoebic potential of Leea indica and its constituent gallic acid in different concentrations. Water and butanol fractions exhibited significant amoebicidal activity against trophozoites and cysts. Gallic acid (100 mu g/mL) revealed 83% inhibition of trophozoites and 69% inhibition of cysts. The butanol fraction induced apoptosis in trophozoites, which was observed using tunnel assay. The cytotoxicity of the fractions and gallic acid was investigated against MRC-5 and no adverse effects were observed. Gallic acid was successfully loaded within poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles with 82.86% encapsulation efficiency, while gallic acid showed 98.24% in vitro release at 48hours. Moreover, the gallic acid encapsulated in the PLGA nanoparticles exhibited 90% inhibition against trophozoites. In addition, gallic acid encapsulated nanoparticles showed reduced cytotoxicity towards MRC-5 compared to gallic acid, which evidenced that natural product nanoencapsulation in polymeric nanoparticles could play an important role in the delivery of natural products.



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Science & Technology - Other Topics


Mahboob, T; Nawaz, M; Pereira, MD; Tian-Chye, T; Samudi, C; Sekaran, SD; Wiart, C; Nissapatorn, V

our authors


Muhammad Nawaz is thankful to Deanship of Scientific Research (DSR), Imam Abdulrahman Bin Faisal University, for financial support through project no. 2016-093 IRMC. This research was supported by a student grant from University of Malaya (PG088-2015A), FRGS/1/2018/WAB07/UNIM/02/1, The project CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/ MCTES, and The project entitled Medicinal underexploited Thai native plants against Acanthamoeba, Leishmania donovani, and Plasmodium falciparum - Toward South East Asia collaboration initiative under The Royal Patronage of Her Royal Highness Princess Maha Chakri Sirindhorn (Grant No. 040226).

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