One-Step All-Aqueous Interfacial Assembly of Robust Membranes for Long-Term Encapsulation and Culture of Adherent Stem/Stromal Cells
authors Vilabril, S; Nadine, S; Neves, CMSS; Correia, CR; Freire, MG; Coutinho, JAP; Oliveira, MB; Mano, JF
nationality International
author keywords all‐ aqueous fabrication; aqueous two‐ phase systems; cell‐ laden capsules; interfacial complexation
abstract The therapeutic effectiveness and biological relevance of technologies based on adherent cells depend on platforms that enable long-term culture in controlled environments. Liquid-core capsules have been suggested as semipermeable moieties with spatial homogeneity due to the high mobility of all components in their core. The lack of cell-adhesive sites in liquid-core structures often hampers their use as platforms for stem cell-based technologies for long-term survival and cell-directed self-organization. Here, the one-step fast formation of robust polymeric capsules formed by interfacial complexation of oppositely charged polyelectrolytes in an all-aqueous environment, compatible with the simultaneous encapsulation of mesenchymal stem/stromal cells (MSCs) and microcarriers, is described. The adhesion of umbilical cord MSCs to polymeric microcarriers enables their aggregation and culture for more than 21 days in capsules prepared either manually by dropwise addition, or by scalable electrohydrodynamic atomization, generating robust and stable capsules. Cell aggregation and secretion overtime can be tailored by providing cells with static or dynamic (bioreactor) environments.
publisher WILEY
issn 2192-2640
isbn 2192-2659
year published 2021
volume 10
issue 10
digital object identifier (doi) 10.1002/adhm.202100266
web of science category 11
subject category Engineering, Biomedical; Nanoscience & Nanotechnology; Materials Science, Biomaterials
unique article identifier WOS:000632240400001
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journal impact factor 7.367
5 year journal impact factor 6.964
category normalized journal impact factor percentile 87.158
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