authors |
Maciel, MM; Correia, TR; Gaspar, VM; Rodrigues, JMM; Choi, IS; Mano, JF |
nationality |
International |
journal |
ADVANCED FUNCTIONAL MATERIALS |
author keywords |
advanced cell‐ material interactions; cell partial coatings; cell‐ based materials; organic– inorganic hybrid composites; sol– gel processes |
abstract |
The manipulation of cell surfaces in anchorage-dependent cells can help overcome difficulties presented during cell handling and application. Silica-based protective mechanisms existing in free-floating microorganisms may be used as inspiration for sustaining human cell survival in suspension. Gathering on this, herein human adipose-derived mesenchymal stem cells (hASCs) are partially coated with a hard silica layer that operates as a supporting platform for individual cells in suspension. Inspired by the organic templates involved in biosilicification, a novel chitosan (CHT) derivative displaying fully natural quaternary amine moieties is synthetized via a rapid, one-pot strategy. Silicification is promoted on individual hASCs surfaces via a two-step process: a priming step onto previously adhered cell with this CHT derivative, followed by a biocompatible sol-gel process. hASCs holding a silica backpack exhibit enhanced cell survival in suspension conditions and can spread and acquire a more adherent phenotype. This new protocol for cell-surface modification also provides a new generation of hybrid materials with functionalization of the silica backpack, which can be applied to different areas such as tissue engineering, biosensing, drug delivery, and targeted cell-based therapies. |
publisher |
WILEY-V C H VERLAG GMBH |
issn |
1616-301X |
isbn |
1616-3028 |
year published |
2021 |
volume |
31 |
issue |
29 |
digital object identifier (doi) |
10.1002/adfm.202009619 |
web of science category |
9 |
subject category |
Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter |
unique article identifier |
WOS:000626638700001
|