Biodistribution and pulmonary metabolic effects of silver nanoparticles in mice following acute intratracheal instillations
authors Rosario, F; Duarte, IF; Pinto, RJB; Santos, C; Hoet, PHM; Oliveira, H
nationality International
journal ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
author keywords Silver nanoparticles; In vivo; Intratracheal instillation; Biodistribution; Excretion; NMR metabolomics
keywords LUNG; TOXICITY; INHALATION; RESPONSES; EXPOSURE; CITRATE; SIZE; CELL; BIOAVAILABILITY; TRANSLOCATION
abstract The respiratory tract is the route of entry for accidentally inhaled AgNPs, which can reach the lungs and redistribute to other main organs through systemic circulation. In the present work, we aimed to evaluate silver biodistribution and biological effects after 1 or 2 intratracheal instillations (IT) of two differently sized PVP-coated AgNPs (5 and 50 nm-3 mg/kg) and ionic silver (AgNO3-1 mg/kg bw) in mice. Furthermore, nuclear magnetic resonance (NMR) metabolomics was applied to unveil pulmonary metabolic variations. Animals exposed to 5 nm AgNP (AgNP5) showed higher levels of ionic silver in organs, especially in the lung, spleen, kidney and liver, while animals exposed to 50 nm AgNP (AgNP50) showed higher levels of silver in the blood. Animals exposed to AgNP50 excreted higher amounts of silver than those exposed to AgNP5, which is consistent with higher tissue accumulation of silver in animals exposed to the latter. Lung metabolic profiling revealed several Ag-induced alterations in metabolites involved in different pathways, such as glycolysis and tricarboxylic acid (TCA) cycle, amino acid and phospholipid metabolism, and antioxidant defense. Notably, most of the metabolic changes observed after 1 IT were absent in animals subjected to 2 IT of AgNO3, or reversed for AgNPs, suggesting adaptation mechanisms to cope with the initial insult and recover homeostasis. Graphical abstract
publisher SPRINGER HEIDELBERG
issn 0944-1344
isbn 1614-7499
year published 2021
volume 28
issue 2
beginning page 2301
ending page 2314
digital object identifier (doi) 10.1007/s11356-020-10563-z
web of science category 14
subject category Environmental Sciences
unique article identifier WOS:000565841700007
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journal impact factor 3.056
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