CRISPR-Cas Technology: Emerging Applications in Clinical Microbiology and Infectious Diseases
authors Serajian, S; Ahmadpour, E; Oliveira, SMR; Pereira, MD; Heidarzadeh, S
nationality International
journal PHARMACEUTICALS
author keywords CRISPR; gene editing; infectious diseases; antimicrobial resistance; parasitology
keywords SEQUENCE-SPECIFIC ANTIMICROBIALS; HORIZONTAL GENE-TRANSFER; NUCLEIC-ACID DETECTION; ISOTHERMAL AMPLIFICATION; CRYSTAL-STRUCTURE; DNA CLEAVAGE; GUIDE RNA; GENOME; REPEATS; SYSTEM
abstract Through the years, many promising tools for gene editing have been developed including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), CRISPR-associated protein 9 (Cas9), and homing endonucleases (HEs). These novel technologies are now leading new scientific advancements and practical applications at an inimitable speed. While most work has been performed in eukaryotes, CRISPR systems also enable tools to understand and engineer bacteria. The increase in the number of multi-drug resistant strains highlights a necessity for more innovative approaches to the diagnosis and treatment of infections. CRISPR has given scientists a glimmer of hope in this area that can provide a novel tool to fight against antimicrobial resistance. This system can provide useful information about the functions of genes and aid us to find potential targets for antimicrobials. This paper discusses the emerging use of CRISPR-Cas systems in the fields of clinical microbiology and infectious diseases with a particular emphasis on future prospects.
publisher MDPI
isbn 1424-8247
year published 2021
volume 14
issue 11
digital object identifier (doi) 10.3390/ph14111171
web of science category 23
subject category Chemistry, Medicinal; Pharmacology & Pharmacy
unique article identifier WOS:000724426900001
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journal analysis (jcr 2019):
journal impact factor 4.286
5 year journal impact factor Not Available
category normalized journal impact factor percentile 82.037
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