abstract
Through the years, many promising tools for gene editing have been developed including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), CRISPR-associated protein 9 (Cas9), and homing endonucleases (HEs). These novel technologies are now leading new scientific advancements and practical applications at an inimitable speed. While most work has been performed in eukaryotes, CRISPR systems also enable tools to understand and engineer bacteria. The increase in the number of multi-drug resistant strains highlights a necessity for more innovative approaches to the diagnosis and treatment of infections. CRISPR has given scientists a glimmer of hope in this area that can provide a novel tool to fight against antimicrobial resistance. This system can provide useful information about the functions of genes and aid us to find potential targets for antimicrobials. This paper discusses the emerging use of CRISPR-Cas systems in the fields of clinical microbiology and infectious diseases with a particular emphasis on future prospects.
keywords
SEQUENCE-SPECIFIC ANTIMICROBIALS; HORIZONTAL GENE-TRANSFER; NUCLEIC-ACID DETECTION; ISOTHERMAL AMPLIFICATION; CRYSTAL-STRUCTURE; DNA CLEAVAGE; GUIDE RNA; GENOME; REPEATS; SYSTEM
subject category
Chemistry, Medicinal; Pharmacology & Pharmacy
authors
Serajian, S; Ahmadpour, E; Oliveira, SMR; Pereira, MD; Heidarzadeh, S
our authors
Maria de Lourdes Gomes Pereira
Associate Professor with Aggregation
Sónia M. R. Oliveira
Junior Researcheracknowledgements
We would like to thank of Muge Cevik and Firooz Shahrivar for their kindly collaboration to perform this work. M.d.L.P. thanks Project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020, national funds by FCT/MCTES.