Grape pectic polysaccharides stabilization of anthocyanins red colour: Mechanistic insights
authors Fernandes, A; Raposo, F; Evtuguin, DV; Fonseca, F; Ferreira-da-Silva, F; Mateus, N; Coimbra, MA; de Freitas, V
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Anthocyanins; Flavylium cation stabilization; Grape pectic polysaccharides; Isothermal titration calorimetry; Malvidin-3-O-beta-D glucoside; UV-visible
keywords BERRY CELL-WALLS; STRUCTURAL-CHARACTERIZATION; KINETIC-PROPERTIES; L.; POLYPHENOLS; BINDING; POMACE; STABILITY; POLYMERS; SULFATE
abstract Grape pectic polysaccharides-malvidin 3-O-beta-D-glucoside binding was studied, aiming to unveil the impact of structural diversity of polysaccharides on anthocyanins-polysaccharides interactions. Polysaccharides were extracted with solutions of imidazole (ISP) and carbonate at 4 degrees C (CSP-4 degrees C) and room temperature (CSP-RT) and also recovered from the dialysis supernatant of the remaining cellulosic residue after the aqueous NAOH extraction of hemicellulosic polysaccharides (Sn-CR). Polysaccharides richer in homogalacturonan domains, like those present in the CSP-4 degrees C fraction had approximately 50-fold higher binding affinity to malvidin-3-O- beta-D-glucoside, than polysaccharides with side chains (as ISP and CSP-RT extractable polysaccharides). CSP-4 degrees C polysaccharides showed a positive effect on malvidin-3-O beta-D-glucoside colour fading. Hydration equilibrium constant of malvidin-3-O- beta-D-glucoside in the presence of CSP-4 degrees C polysaccharides was higher, showing the preferential stabilization of the flavylium cation. The results showed that anthocyanins colour stabilization can be promoted by pectic polysaccharide structures such as those extracted by cold carbonate.
publisher ELSEVIER SCI LTD
issn 0144-8617
isbn 1879-1344
year published 2021
volume 255
digital object identifier (doi) 10.1016/j.carbpol.2020.117432
web of science category 10
subject category Chemistry, Applied; Chemistry, Organic; Polymer Science
unique article identifier WOS:000608737100008
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journal analysis (jcr 2019):
journal impact factor 7.182
5 year journal impact factor 6.89
category normalized journal impact factor percentile 96.638
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