Improvement of carotenoids production from Rhodotorula glutinis CCT-2186


Rhodotorula strains can produce industrial valuable bioproducts. In this work, the production of carotenoids, and lipids by Rhodotorula glutinis using different nitrogen sources was evaluated. Two statistical experimental designs were applied to improve carotenoid production: a first 2(S-1) fractional factorial design evaluating the influence of independent variables pH, nitrogen source, glucose, KH2PO4 and MgSO4 concentration; a second 2(2) central factorial design to optimize the effect of pH and nitrogen sources. After the optimization using two statistical designs, a culture mediium composed of (in g/L) glucose (10), asparagine (10), NH4NO3 (4), KH2PO4 (0.52), MgSO 4 (0.52) was found as the best for the production of carotenoids at a pH 5 and 30 degrees C. The best bioprocess was scaled-up to a 5 L stirred-tank bioreactor. The change to a bioreactor allowed to improve aeration and agitation conditions, and consequently, increasing the production yields (m/v) in, approximately, 25.83 %, 11.88 %, 24.50 % and 10.32 % for beta-carotene, torularhodin, torulene and lipids, respectively. The combined supplementation of the culture media with both organic (asparagine) and inorganic nitrogen (ammonium nitrate) sources was primordial for enhancing the carotenogenesis. R. glutinis are very efficient in the production of valuable carotenoids and lipids, presenting high potential of yeast for the industrial production of more sustainable cosmetic, pharmaceutical, and food products.



subject category

Biotechnology & Applied Microbiology; Engineering, Chemical


Mussagy, CU; Guimaraes, AAC; Rocha, LVF; Winterburn, J; Santos-Ebinuma, VD; Pereira, JFB

our authors



This research was supported by grants from the Ministry of Science and Technology, High Education and Technical Vocational Training of Mozambique (MCTESTP, Maputo, Mozambique)through the HEST Project-World Bank and the FAPESP (Sao Paulo Research Foundation, Brazil) through the projects: 2014/19793-3; 2014/16424-7; 2014/01580-3; 2017/50303-0 and 2019/09618-3. C.U. Mussagy also acknowledges the support from the CAPES-PRINT (Coordination of Superior Level Staff Improvement, Brazil)-Finance Code 88887.367977/2019-00 and CAPES-PROEX-Finance code 001.

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