Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

abstract

The extracellular matrix plays a critical role in bioinstructing cellular self-assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next-generation tissue-specific hydrogels with close-to-native biomolecular signatures and user-tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue-specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom-up cell engineering technologies may unravel unprecedented tissue-like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient-tailored living therapeutics.

keywords

EXTRACELLULAR-MATRIX HYDROGELS; HUMAN AMNIOTIC MEMBRANE; PLURIPOTENT STEM-CELLS; IN-VITRO; COLORECTAL-CANCER; INTESTINAL ORGANOIDS; RETINAL ORGANOIDS; CULTURE-SYSTEM; LUNG; LIVER

subject category

Chemistry; Science & Technology - Other Topics; Materials Science; Physics

authors

Moura, BS; Monteiro, MV; Ferreira, LP; Lavrador, P; Gaspar, VM; Mano, JF

our authors

acknowledgements

This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). This work was also supported by the Programa Operacional Competitividade e Internacionalizacao (POCI), in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the projects PANGEIA (PTDC/BTM-SAL/30503/2017). The authors also acknowledge the support of the European Research Council (ERC) for project REBORN (grant agreement ERC-H20202019-ADG-883370). The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through a Doctoral Grant (2021.08331.BD, B.S.M.) and through a Junior Researcher contract (CEEC/1048/2019, V.M.G.).

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