resumo
The extracellular matrix plays a critical role in bioinstructing cellular self-assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next-generation tissue-specific hydrogels with close-to-native biomolecular signatures and user-tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue-specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom-up cell engineering technologies may unravel unprecedented tissue-like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient-tailored living therapeutics.
palavras-chave
EXTRACELLULAR-MATRIX HYDROGELS; HUMAN AMNIOTIC MEMBRANE; PLURIPOTENT STEM-CELLS; IN-VITRO; COLORECTAL-CANCER; INTESTINAL ORGANOIDS; RETINAL ORGANOIDS; CULTURE-SYSTEM; LUNG; LIVER
categoria
Chemistry; Science & Technology - Other Topics; Materials Science; Physics
autores
Moura, BS; Monteiro, MV; Ferreira, LP; Lavrador, P; Gaspar, VM; Mano, JF
nossos autores
Beatriz Silva Moura
Estudante de doutoramento
João Mano
Vice-Diretor
Luís Pedro Correia Pinto Ferreira
Estudante de doutoramento
Maria Vinhas Monteiro
Estudante de doutoramento
Pedro Lavrador
Estudante de doutoramento
Vítor Gaspar
Professor AuxiliarProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). This work was also supported by the Programa Operacional Competitividade e Internacionalizacao (POCI), in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the projects PANGEIA (PTDC/BTM-SAL/30503/2017). The authors also acknowledge the support of the European Research Council (ERC) for project REBORN (grant agreement ERC-H20202019-ADG-883370). The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through a Doctoral Grant (2021.08331.BD, B.S.M.) and through a Junior Researcher contract (CEEC/1048/2019, V.M.G.).