Antibacterial activity improvement of dental glass- ceramic by incorporation of AgVO3 nanoparticles


Objective: This study aimed to investigate the role of the incorporation of an antibacterial nanoceramic (AgVO3) on the properties of a restorative dental glass-ceramic.Method: A commercially available restorative glass-ceramic, commonly designated as porcelain (IPS d.SIGN) was functionalized with an antibacterial agent (nanostructured 8-AgVO3), synthesized by a hydrothermal route. Both functionalized and pristine samples were processed according to the manufacturer's instructions. All samples were char-acterized by X-ray diffraction, Rietveld refinement, particle size distribution, Scanning Electron Microscopy, chemical solubility, and Inductively Coupled Plasma Spectroscopy. Their antibacterial potential (Mueller-Hinton test) was analyzed against gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli).Results: The commercial glass-ceramic showed leucite (KAlSi2O6) as the only detectable crystalline phase, and, for both strains, no antibacterial activity could be detected in the Mueller-Hinton agar plates test. A monophasic, needle-shaped, and nanometric 8-AgVO3 powder was successfully synthesized by a simple hydrothermal route. After thermal treatment, glass-ceramic samples containing different percentages of 8-AgVO3 showed a second crystalline phase of microline [K0.95(AlSi3O8)]. For modified samples, inhibition halos were easily visible on the Mueller-Hinton test, which ranged from 11.1 +/- 0.5 mm to 16.6 +/- 0.5 mm and 12.7 +/- 0.3 mm to 15.5 +/- 0.3 mm in the S. aureus and E.coli cultures, respectively, showing that the halos formed were dose-dependent. Also, increasing the percentage of 8-AgVO3 promoted a significant increase in chemical solubility, from 72 1.1.g/ cm2 (samples with 1 wt% of 8-AgVO3) to 136 1.1.g/cm2 (samples with 2 wt% of 8-AgVO3), which was associated with the silver and vanadium ions released from the glass matrix.Significance: Our in vitro results indicate that IPS d.SIGN, as most of the dental glass-ceramics, do not exhibit antibacterial activity per se. Nonetheless, in this concept test, we demonstrated that it is possible to modify dental veneering materials giving them antibacterial properties by adding at least 2 wt% of beta-AgVO3, a nanomaterial easily synthesized by a simple route.(c) 2022 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.



subject category

Dentistry, Oral Surgery & Medicine; Materials Science


Baptista, IO; Alves, MFRP; Ferreira, S; Santos, C; Vieira, SI; Fernandes, MHV

our authors


This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. Financial support by the grant BD/06615/2021 from PT national funds (FCT/MCTES, Fundacao para a Ciencia e Tecnologia and Ministerio da Ciencia, Tecnologia e Ensino Superior) is acknowledged.

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