Bioactive Bacterial Nanocellulose Membranes Enriched with Eucalyptus globulus Labill. Leaves Aqueous Extract for Anti-Aging Skin Care Applications


Bacterial nanocellulose (BNC) membranes, with remarkable physical and mechanical properties, emerged as a versatile biopolymeric carrier of bioactive compounds for skin care applications. In this study, BNC membranes were loaded with glycerol (as plasticizer and humectant agent) and different doses (1-3 mu g cm(-2)) of an aqueous extract obtained from the hydro-distillation of Eucalyptus globulus Labill. leaves (HDE), for application as sheet facial masks. All membranes are resistant and highly malleable at dry and wet states, with similar or even better mechanical properties than those of a commercial BNC mask. Moreover, the HDE was found to confer a dose-dependent antioxidant activity to pure BNC. Additionally, upon 3 months of storage at 22-25 degrees C and 52% relative humidity (RH) or at 40 degrees C and 75% RH, it was confirmed that the antioxidant activity and the macroscopic aspect of the membrane with 2 mu g cm(-2) of HDE were maintained. Membranes were also shown to be non-cytotoxic towards HaCaT and NIH/3T3 cells, and the membrane with 2 mu g cm(-2) of HDE caused a significant reduction in the senescence-associated beta-galactosidase activity in NIH/3T3 cells. These findings suggest the suitability and potential of the obtained membranes as bioactive facial masks for anti-aging applications.



subject category

Chemistry; Materials Science; Metallurgy & Metallurgical Engineering; Physics


Almeida, T; Moreira, P; Sousa, FJ; Pereira, C; Silvestre, AJD; Vilela, C; Freire, CSR

our authors


This work was carried out under the project Inpactus (Innovative Products and Technologies from Eucalyptus), project no. 21874, funded by Portugal 2020 through the European Regional Development Fund (ERDF) in the frame of COMPETE 2020 no. 246/AXIS II/2017, and within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). Fundacao para a Ciencia e a Tecnologia (FCT) is also acknowledged for the research contract under Scientific Employment Stimulus to C.V. (CEECIND/00263/2018 and 2021.01571.CEECIND) and C.S.R.F. (CEECIND/00464/2017).

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