Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation

abstract

The emergence of biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, revolutionized the medical field, contributing to significant advances in the prophylaxis and treatment of chronic and life-threatening diseases. However, biopharmaceuticals manufacturing involves a set of complex upstream and downstream processes, which considerably impact their cost. In particular, despite the efforts made in the last decades to improve the existing technologies, downstream processing still accounts for more than 80% of the total biopharmaceutical production cost. On the other hand, the formulation of biological products must ensure they maintain their therapeutic performance and long-term stability, while preserving their physical and chemical structure. Ionic-liquid (IL)-based approaches arose as a promise alternative, showing the potential to be used in downstream processing to provide increased purity and recovery yield, as well as excipients for the development of stable biopharmaceutical formulations. This manuscript reviews the most important progress achieved in both fields. The work developed is critically discussed and complemented with a SWOT analysis.

keywords

AQUEOUS 2-PHASE SYSTEMS; SOLID-PHASE EXTRACTION; HUMAN SERUM-ALBUMIN; IMMUNOGLOBULIN-G; CONFORMATIONAL STABILITY; ANTIBODY PURIFICATION; MONOCLONAL-ANTIBODIES; SELECTIVE ISOLATION; CHEMICAL-STABILITY; BIPHASIC SYSTEMS

subject category

Biotechnology & Applied Microbiology; Engineering

authors

Almeida, C; Pedro, AQ; Tavares, APM; Neves, MC; Freire, MG

our authors

acknowledgements

This work is financed by Portugal 2020 through European Regional Development Fund (ERDF) in the frame of CENTRO2020 in the scope of the project AntYmicrob, CENTRO -01-0247-FEDER-181219 and in the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020 and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). This work was additionally developed within the scope of the EIC-Pathfinder YSCRIPT project with reference 101047214, supported by the budgets of the Horizon Europe Program.

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