abstract
BACKGROUND: When the human population increases, so does the need to explore a wider range of feedstocks and biomasses, such as cyanobacteria. However, a deeper understanding of the growth patterns and pigment production is required to support the selection of the most beneficial species and conditions for industrial production. The growth and pigment production (i.e., chlorophyll a and C-phycocyanin) of three cyanobacterium species were evaluated following a three-fold aim. The first goal was to compare among a species commonly selected for exploitation (Arthrospira platensis) and two alternative species (Anabaena cylindrica and Nostoc muscorum). The second goal was analyzing pigment production in the long-term. The last goal involved comparing different methods (spectrophotometry and fluorimetry) to understand whether there is an appropriate proxy of biomass increase and pigment production that can be used for monitoring purposes. RESULTS: All species showed high longevity and proved capable of growing for more than 100 days without any additional supplementation. However, the maximum quantum yield of PS II (F-v /F-m) revealed that their photosynthetic efficiency varied over time with a clear decrease after 2 months. Pigment analysis showed a heterogeneous pattern during the growth periods of all three species that could only be captured by the parameter F-v /F-m, but the pattern was only present for A. cylindrica and N. muscorum in some stages of the culture period. CONCLUSION: N. muscorum was found to be the best chlorophyll a and C-phycocyanin producer, with the production peaking for all species at defined time periods within the growth profile. (C) 2022 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
keywords
CHLOROPHYLL FLUORESCENCE; MICROPHYTOBENTHIC BIOMASS; C-PHYCOCYANIN; GROWTH; PHOTOSYNTHESIS; PIGMENT; PHYCOBILIPROTEINS; ANTIOXIDANT; MICROALGAE
subject category
Biotechnology & Applied Microbiology; Chemistry; Engineering
authors
Macário, IPE; Veloso, T; Fernandes, APM; Martins, M; Frankenbach, S; Serôdio, J; Gonçalves, FJM; Ventura, SPM; Pereira, JL
our authors
Inês Adriana Patrunilho Efe Macário
PhD Student
Sónia Patrícia Marques Ventura
Assistant ProfessorProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
acknowledgements
Thanks are due to FCT/MCTES for the financial support to CESAM (UIDB/50017/2020+UIDP/50017/2020+LA/P/0094/2020) and CICECO (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020) through national funds. This work was supported by the project REFINACYANO (PTDC/BTA-BTA/30914/2017), and EVOSYM (POCI-01-0145-FEDER-028751) funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalizacao (POCI), and by national funds (OE) through FCT/MCTES. I.P.E. Macario (SFRH/BD/123850/2016), T. Veloso (SFRH/BD/147346/2019) and M. Martins (SFRH/BD/122220/2016) thank FCT for their doctoral grants.