abstract
Extended-spectrum beta-lactamase (ESBL) production and biofilm formation are mechanisms employed by Escherichia coli to resist beta-lactam antibiotics. Thus, we aimed to examine antibiotic resistance associated with ESBL production and biofilm formation in E. coli isolates from swine farms in Southern Thailand. In total, 159 E. coli isolates were obtained, with 44 isolates identified as ESBL producers, originating from feces (18.87 %) and wastewater (8.80 %) samples. All ESBL-producing strains exhibited resistance to ampicillin (100 %), followed by the cephalosporin group (97.73 %) and tetracycline (84.09 %). Multidrug resistance was observed in 17 isolates (38.63 %). Among the isolates from feces samples, the blaGES gene was the most prevalent, detected in 90 % of the samples, followed by blaCTX-M9 (86.67 %) and blaCTX-M1 (66.67 %), respectively. In the bacteria isolated from wastewater, both blaGES and blaCTX-M9 genes were the predominant resistance genes, detected in 100 % of the isolates, followed by blaCTX-M1 (64.29 %) and blaTEM (50 %), respectively. Strong biofilm formation was observed in 11 isolates (36.67 %) from feces and 4 isolates (25.57 %) from wastewater samples. Notably, nearly 100 % of ESBL-producing strains isolated from feces tested positive for both pgaA and pgaC genes, which play a role in intracellular adhesion and biofilm production. These findings contribute to the understanding and potential control of ESBL-producing E. coli, and the dissemination of antibiotic resistance and biofilm-related genes in swine farms.
keywords
ANTIMICROBIAL RESISTANCE; MANURE; GENES; MEAT
subject category
Immunology; Microbiology; Veterinary Sciences
authors
Mitsuwan, W; Intongead, S; Saengsawang, P; Romyasamit, C; Narinthorn, R; Nissapatorn, V; Pereira, MD; Paul, AK; Wongtawan, T; Boripun, R
our authors
Maria de Lourdes Gomes Pereira
Associate Professor with Aggregationacknowledgements
This work was funded by Walailak University's Individual Research Grant, Walailak University (Grant number; WU-IRG-65-008), Center of Excellence in Innovation of Essential Oil and Bioactive Compounds (Grant no. WU-COE-65-05), Thailand Science Research and Innovation Fund (Contract No. FRB650082/0227), Project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/ 2020 and was financed by national funds via the FCT/MEC (PIDDAC).