abstract
3D bioprinting isa versatile technique that allows the fabricationof living tissue analogs through the layer-by-layer deposition ofcell-laden biomaterials, viz. bioinks. In this work, composite alginatehydrogel-based bioinks reinforced with curcumin-loaded particles ofcellulose esters (CEpCUR) and laden with human keratinocytes (HaCaT)are developed. The addition of the CEpCUR particles, with sizes of740 & PLUSMN; 147 nm, improves the rheological properties of the inks,increasing their shear stress and viscosity, while preserving therecovery rate and the mechanical and viscoelastic properties of theresulting fully cross-linked hydrogels. Moreover, the presence ofthese particles reduces the degradation rate of the hydrogels from26.3 & PLUSMN; 0.8% (ALG) to 18.7 & PLUSMN; 1.3% (ALG:CEpCUR_10%) after3 days in the culture medium. The 3D structures printed with the ALG:CEpCURinks reveal increased printing definition and the ability to releasecurcumin (with nearly 70% of cumulative release after 24 h in PBS).After being laden with HaCaT cells (1.2 x 10(6) cellsmL(-1)), the ALG:CEpCUR bioinks can be successfully3D bioprinted, and the obtained living constructs show good dimensionalstability and high cell viabilities at 7 days post-bioprinting (nearly90%), confirming their great potential for application in fields likewound healing.
keywords
SUSTAINED-RELEASE; BIO-INKS; 3D; NANOPARTICLES; STABILITY; MEMBRANES; SCAFFOLD
subject category
Science & Technology - Other Topics; Materials Science
authors
Carvalho, JPF; Teixeira, MC; Lameirinhas, NS; Matos, FS; Luís, JL; Pires, L; Oliveira, H; Oliveira, M; Silvestre, AJD; Vilela, C; Freire, CSR
our authors
Armando Jorge Domingues Silvestre
Full professor
Carla Vilela
Assistant Professor
Carmen Sofia da Rocha Freire Barros
Coordinating Researcher
Helena Oliveira
Post-Doc Fellowship
J. Martinho Oliveira
Coordinator Professor
Jorge Louro Luís
PhD Student
Liliana Sofia Oliveira Pires
Adjunct ProfessorGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
Projects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
This work was developed within the scope of the projects CICECO-Aveiro Institute of Materials (UIDB/50011/2020, UIDP/50011/2020. LA/P/0006/2020) and CESAM (UIDP/50017/2020. UIDB/50017/2020. LA/P/0094/2020), financed by national funds through the FCT/MCTES (PIDDAC), and financially supported by the project I & amp;D "NANOBIOINKS- Engineering bio-based nanofibers for the development of high-performance nanostructured bioinks for 3-D bioprinting, CENTRO-01-0145-FEDER-031289" funded by the Operational Program of the Center Region, in its FEDER/FNR component, and by national funds (OE), through FCT/MCTES. FCT is acknowledged for the doctoral grants to J.P.F.C (2020.09018.BD) and N.S.L. (SFRH/BD/140229/2018) and the research contracts under Scientific Employment Stimulus to C.S.R.F. (CEECIND/00464/2017), C.V. (CEECIND/00263/2018 and 2021.01571.CEECIND) and H.O. (CEECIND/04050/2017).