abstract
An increase of multi-drug resistant Escherichia coli in swine husbandry have been concerned worldwide. This study aimed to determine the multidrug resistance and nucleotide substitution of beta-lactam antibiotic and tetracycline resistant genes in E. coli from swine farms in Southern Thailand. A total of 112 isolates of E. coli was isolated from 50 pig farms, which were confirmed by identified by MALDI-TOF analysis. Seventy-three isolates (65.18%) and 39 isolates (34.82%) were isolated from the feces and waste water samples, respectively. One hundred percent resistance to beta-lactam antibiotics as well as their resistant gene blaTEM was detected in isolates. Furthermore, 81% isolates were tetracycline resistance and both tetA [68.42% (13/19) in feces samples, 72.73% (8/11) in waste water samples] and tetB [10.53% (2/19) in feces samples, 18.18% (2/11) in waste water samples] genes responsible for tetracycline resistance were observed. Furthermore, 54 isolates had multi-drug resistance that presented 11 different patterns. The nucleotide substitution of genes was detected in 3 isolates of E. coli, and may consider as the point mutation. The nucleotide at 859 bp of tetA gene of the isolate WU-WW009-01 was changed from T to A. While, the isolate WU-WW004-02 showed 2 nucleotide substitution sites at the position of 266 (from A to G) and 859 (from T to G) bp. The nucleotide at 36 bp of blaTEM gene of the isolate WU-F003-02 was replaced from G to A. Findings of this study may help to control the spread of E. coli antibiotic resistance genes in the swine farms.& COPY; 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
subject category
Environmental Sciences & Ecology; Toxicology
authors
Boripun, R; Saengsawang, P; Intongead, S; Narinthorn, R; Wongtawan, T; Nissapatorn, V; Pereira, MD; Mitsuwan, W
our authors
Maria de Lourdes Gomes Pereira
Associate Professor with AggregationProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
This work was funded by Center of Excellence in Innovation of Essential Oil and Bioactive Compounds (Grant no. WU-COE-65-05), Thailand Science Research and Innovation Fund (Contract No. FRB650082/0227-WU07). We would like to thank One Health Research Center, Walailak University, Thailand, for the support, and Project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC).