Assessment of yeast viability under hyperbaric conditions through a modeling approach


The effect of pressure (0.1-1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining method and the percentages of viable and non-viable cells were estimated using digital image processing. A model taking into account cell viability was developed and used to describe the measured data. The model reveals the opposing effects between oxygen availability and the baric and oxidative stresses present on the system and can successfully describe not only the traditional biomass-product-substrate (X-P-S) evolution but also the ratio of viable cells with time. It is shown that cell viability in general is not constant during the experiments but strongly depends on the environment. (c) 2005 Society of Chemical Industry.



subject category

Biotechnology & Applied Microbiology; Chemistry; Engineering


Coutinho, JAP; Belo, I; Ferreira, EC; Coelho, MAZ

our authors

Share this project:

Related Publications

We use cookies for marketing activities and to offer you a better experience. By clicking “Accept Cookies” you agree with our cookie policy. Read about how we use cookies by clicking "Privacy and Cookie Policy".