Assessment of yeast viability under hyperbaric conditions through a modeling approach
authors Coutinho, JAP; Belo, I; Ferreira, EC; Coelho, MAZ
nationality International
journal JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
author keywords modeling; digital image processing; cell viability; yeast
keywords SACCHAROMYCES-CEREVISIAE; STRESS; REACTORS
abstract The effect of pressure (0.1-1.5 MPa) and oxygen concentration on the growth and viability of Saccharomyces cerevisiae was studied. Cell viability was assessed through the Methylene Blue staining method and the percentages of viable and non-viable cells were estimated using digital image processing. A model taking into account cell viability was developed and used to describe the measured data. The model reveals the opposing effects between oxygen availability and the baric and oxidative stresses present on the system and can successfully describe not only the traditional biomass-product-substrate (X-P-S) evolution but also the ratio of viable cells with time. It is shown that cell viability in general is not constant during the experiments but strongly depends on the environment. (c) 2005 Society of Chemical Industry.
publisher JOHN WILEY & SONS LTD
issn 0268-2575
year published 2005
volume 80
issue 8
beginning page 872
ending page 877
digital object identifier (doi) 10.1002/jctb.1248
web of science category Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical
subject category Biotechnology & Applied Microbiology; Chemistry; Engineering
unique article identifier WOS:000230701800003
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journal analysis (jcr 2019):
journal impact factor 2.75
5 year journal impact factor 2.857
category normalized journal impact factor percentile 53.374
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