abstract
In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fibroblast lineage or cells originating from a rat osteosarcoma (ROS 17/2.8) showed a dose dependent decrease in cell proliferation in response to 5-FU-, either dissolved in the culture medium or loaded onto particles. The response of the two cell lines to loaded and nonloaded particles was different. The effect of LG and of a corresponding concentration of free 5-FU was practically the same for the ROS 17/2.8 cells indicating that ROS 17/2.8 cells were not affected by the carrier material. In contrast, L929 cells showed a slight decrease in cell proliferation also in the presence of granules not loaded with 5-FU. This is thought to be attributed to the inhibition of mitogenesis by phosphocitrates, already demonstrated in fibroblasts. In summary, we found that the loaded 5-FU kept its effectivity after the spray drying process and that the response towards the granules varied with cell type. This is the first step towards a tissue specific DDS.
keywords
BASIC CALCIUM-PHOSPHATE; HYDROXYAPATITE NANOPARTICLES; CONTROLLED MORPHOLOGY; PHOSPHOCITRATE; MITOGENESIS; MECHANISMS; PARTICLES; APOPTOSIS; DELIVERY; BEHAVIOR
subject category
Chemistry
authors
Scharnweber, T; Santos, C; Franke, RP; Almeida, MM; Costa, MEV
our authors
Maria Elisabete Jorge Vieira Costa
Collaborator
Maria Margarida Tavares Lopes Almeida
Retired ProfessorGroups
G2 - Photonic, Electronic and Magnetic Materials
G5 - Biomimetic, Biological and Living Materials
acknowledgements
This work was supported in part by the European Project G5RD-CT2000-00282 Tissue Reactor.