Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures
authors Amaral, M; Gomes, PS; Lopes, MA; Santos, JD; Silva, RF; Fernandes, MH
nationality International
journal ACTA BIOMATERIALIA
author keywords Nanocrystalline diamond coating; Cytotoxicity; Fibroblast cell cultures; Silicon nitride; Biomedical dental applications
keywords CHEMICAL-VAPOR-DEPOSITION; HOT-FILAMENT CVD; IN-VITRO; OSTEOBLAST ADHESION; NANOPHASE CERAMICS; SILICON-NITRIDE; ULTRANANOCRYSTALLINE DIAMOND; TEMPOROMANDIBULAR-JOINT; FUNCTIONALIZED DIAMOND; PERIODONTAL-LIGAMENT
abstract The cytotoxicity profile of nanocrystalline diamond (NCD) coatings on a Si(3)N(4) ceramic was investigated. This material is envisaged to have biomedical dental applications such as burrs and surgical instruments. Two fibroblast cell culture systems were used to address the cytotoxicity of NCD-coated samples: L929 cells (a mouse permanent cell line) and human gingival fibroblasts. Cell behavior was evaluated in terms of cell adhesion, cell viability/proliferation (mitochondrial function, MTT assay) and the pattern of cell growth. Fibroblast cell behavior on standard polystyrene culture plates was used as control, as Si3N4 substrates have previously been shown to be biocompatible. NCD coatings provided a suitable surface for cell attachment, spreading and proliferation. Human gingival cells showed a homogeneous cytoplasm spreading, a flattened elongated morphology and a typical parallel alignment on confluent cultures. In comparison, L929 cells denoted a lower cytoplasm expansion, a heterogeneous spreading but a higher proliferation rate. For both cells, after few days, the NCD coating was completely covered with continuous cell layers. As compared to standard polystyrene culture plates, no deleterious or cytotoxic responses were observed with L929 and human fibroblast cell cultures, and in both a slight enhancement in cell proliferation was observed. In addition, the seeded NCD film allowed reproduction of the typical features of the two cell culture systems tested, further suggesting the lack of cytotoxicity of this coating. (c) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 1742-7061
year published 2009
volume 5
issue 2
beginning page 755
ending page 763
digital object identifier (doi) 10.1016/j.actbio.2008.08.015
web of science category Engineering, Biomedical; Materials Science, Biomaterials
subject category Engineering; Materials Science
unique article identifier WOS:000262684600024
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journal analysis (jcr 2019):
journal impact factor 7.242
5 year journal impact factor 7.502
category normalized journal impact factor percentile 89.769
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