Characterization of an extracellular polysaccharide produced by a Pseudomonas strain grown on glycerol

authors	Freitas, F; Alves, VD; Pais, J; Costa, N; Oliveira, C; Mafra, L; Hilliou, L; Oliveira, R; Reis, MAM
nationality	International
journal	BIORESOURCE TECHNOLOGY
author keywords	Extracellular polysaccharide; Glycerol; Pseudomonas oleovorans; Rheology; Biodegradable films
keywords	SOLID-STATE; CELL-WALL; KONJAC GLUCOMANNAN; GLASS-TRANSITION; EXOPOLYSACCHARIDE; SPECTROSCOPY; PULLULAN; CULTURE; STARCH; FILMS
abstract	A new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of Pseudomonas oleovorans NRRL B-14682 using glycerol as the sole carbon source. Thermal and solid-state NMR analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7 degrees C. The exopolysaccharide aqueous solutions have viscoelastic properties similar to that of Guar gum, but with affinity to salts as a result of its polyelectrolyte character. In addition, the exopolysaccharide has demonstrated good flocculating and emulsifying properties and film-forming capacity. These properties make this polymer a good alternative to more expensive natural polysaccharides, such as Guar gum, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries. (C) 2008 Elsevier Ltd. All rights reserved.
publisher	ELSEVIER SCI LTD
issn	0960-8524
year published	2009
volume	100
issue	2
beginning page	859
ending page	865
digital object identifier (doi)	10.1016/j.biortech.2008.07.002
web of science category	Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels
subject category	Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels
unique article identifier	WOS:000261080700053