Effect of the Inducers Veratryl Alcohol, Xylidine, and Ligninosulphonates on Activity and Thermal Stability and Inactivation Kinetics of Laccase from Trametes versicolor
authors Saraiva, JA; Tavares, APM; Xavier, AMRB
nationality International
journal APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
author keywords Laccase production; Inducers; Thermal inactivation; Trametes versicolor; Kinetics
keywords BACILLUS-AMYLOLIQUEFACIENS; PLEUROTUS-OSTREATUS; ENHANCED PRODUCTION; ALPHA-AMYLASE; KRAFT PULP; INDUCTION; DELIGNIFICATION; DECOLORIZATION; PURIFICATION; EFFLUENT
abstract Laccase production from Trametes versicolor was improved in the presence of the inducers ligninosulphonates, veratryl alcohol, and xylidine respectively two-, four-, and eightfold. The thermal inactivation of the produced laccase, after partial purification with ammonium sulfate was kinetically investigated at various temperatures (60-70 A degrees C) and pH values (3.5, 4.5, and 5.5). The inactivation process followed first-order kinetics for all conditions tested, except for veratryl alcohol, for which a constant activity level was observed at the end of the inactivation, also after first-order decay. Enzyme thermostability was affected by the type of inducer used in the culture medium for the production of laccase and also by the pH of incubation mixture. Generally, laccase stability increased with pH increment, being more stable at pH 5.5, except with xylidine. At pHs 4.5 and 5.5, the three inducers significantly increased laccase thermal stability, with the higher effect being observed for pH 5.5 and ligninosulphonates, where increment of half-life times ranged from 3- to 20-fold, depending on the temperature.
publisher HUMANA PRESS INC
issn 0273-2289
year published 2012
volume 167
issue 4
beginning page 685
ending page 693
digital object identifier (doi) 10.1007/s12010-012-9719-3
web of science category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology
unique article identifier WOS:000304930200002
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