abstract
Recently, efforts have been put on the development of new drug formulations using ionic liquid framework. In this work, two different species of abroad-spectrum polyketide antibiotic, tetracycline, are studied in terms of some important properties for antibiotics such as solubility in water and hydrophilichydrophobic balance. Tetracycline was used as cation, whereas docusate, a biocompatible anion, which enables the tailoring of the hydrophilicity of salts, was chosen as the anion. The developed innovative ion pair, tetracycline docusate, was characterized in terms of its thermal stability, water solubility, octanolwater, and liposomewater partition coefficients, using UVvis spectrophotometry because of the absorbance of tetracycline around 270nm. Egg yolk phosphatidylcholine liposomes were used as cell membrane models, and the interactions of both tetracycline hydrochloride and tetracycline docusate with the liposomes were quantified by determination of the partition coefficient using derivative spectrophotometry. A theoretical model based on simple partition drugs between two different media was used to determine the partition coefficient in liposomes. (c) 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:15041512, 2013
keywords
ACTIVE PHARMACEUTICAL INGREDIENTS; HYDROCHLORIDE FORMS; COEFFICIENTS; ANTIBIOTICS; SOLUBILITY; CIPROFLOXACIN; SOLVENTS; SALTS; WATER
subject category
Pharmacology & Pharmacy; Chemistry
authors
Alves, F; Oliveira, FS; Schroder, B; Matos, C; Marrucho, IM
our authors
Bernd Schroder
Post-doc Fellowship
Isabel Maria Delgado Jana Marrucho Ferreira
Full professorGroups
acknowledgements
This work was financed by national funding from FCT-Fundacao para a Ciencia e a Tecnologia, through project PTDC/EQU-EPR/104554/2008. The authors also acknowledge FCT for the grants SFRH/BD/73761/2010 and SFRH/BPD/38637/2007 of F.S. Oliveira and B. Schroder, respectively, and I.M. Marrucho contract under Ciencia 2007 Program. The NMR spectrometers are part of the National NMR Network and were purchased within the framework of the National Program for the Renovation of Scientific Equipment, contract REDE/1517/RMN/2005, with funds from POCI 2010 (FEDER) and FCT/MCTES.