Quantitation of aliphatic suberin in Quercus suber L. cork by FTIR spectroscopy and solid-state C-13-NMR spectroscopy

resumo

This work. determined that the percentage of suberin in cork may be found by solid-state C-13 cross polarization/magic angle spinning (CP/MAS) NMR spectroscopy and by FTIR with photoacoustic detection (FTIR-PAS) spectroscopy. A linear relationship is found between the suberin content measured through CP/MAS spectral areas and that measured gravimetrically. Furthermore, application of a partial least squares (PLS1) regression model to the NMR and gravimetric data sets clearly correlates the two sets, enabling suberin quantification with 90% precision. Suberin quantitation by FTIR-PAS spectroscopy is also achieved by a PLS1 regression model, giving 90% accurate estimates of the percentage of suberin in cork. Therefore, C-13-CP/MAS NMR and FTIR-PAS proved to be useful and accurate noninvasive techniques to quantify suberin in cork, thus avoiding the traditional time consuming and destructive chemical methods. (C) 2000 John Wiley & Sons, Inc.

palavras-chave

NUCLEAR-MAGNETIC-RESONANCE; TRANSFORM INFRARED SPECTROMETRY; POTATO CELL-WALL; LIGNIN; WOOD; REGRESSION; CELLULOSE; POLYMERS; PLANTS; CUTIN

categoria

Biochemistry & Molecular Biology; Biophysics

autores

Lopes, MH; Neto, CP; Barros, AS; Rutledge, D; Delgadillo, I; Gil, AM

nossos autores

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