Quantitation of aliphatic suberin in Quercus suber L. cork by FTIR spectroscopy and solid-state C-13-NMR spectroscopy
authors Lopes, MH; Neto, CP; Barros, AS; Rutledge, D; Delgadillo, I; Gil, AM
journal BIOPOLYMERS
author keywords cork; Quercus suber L.; suberin; solid-state NMR; C-13-cross polarization/magic angle spinning; FTIR spectroscopy with photoacoustic detection; quantitation; multivariate calibration
keywords NUCLEAR-MAGNETIC-RESONANCE; TRANSFORM INFRARED SPECTROMETRY; POTATO CELL-WALL; LIGNIN; WOOD; REGRESSION; CELLULOSE; POLYMERS; PLANTS; CUTIN
abstract This work. determined that the percentage of suberin in cork may be found by solid-state C-13 cross polarization/magic angle spinning (CP/MAS) NMR spectroscopy and by FTIR with photoacoustic detection (FTIR-PAS) spectroscopy. A linear relationship is found between the suberin content measured through CP/MAS spectral areas and that measured gravimetrically. Furthermore, application of a partial least squares (PLS1) regression model to the NMR and gravimetric data sets clearly correlates the two sets, enabling suberin quantification with 90% precision. Suberin quantitation by FTIR-PAS spectroscopy is also achieved by a PLS1 regression model, giving 90% accurate estimates of the percentage of suberin in cork. Therefore, C-13-CP/MAS NMR and FTIR-PAS proved to be useful and accurate noninvasive techniques to quantify suberin in cork, thus avoiding the traditional time consuming and destructive chemical methods. (C) 2000 John Wiley & Sons, Inc.
publisher JOHN WILEY & SONS INC
issn 0006-3525
year published 2000
volume 57
issue 6
beginning page 344
ending page 351
digital object identifier (doi) 10.1002/1097-0282(2000)57:6<344::AID-BIP40>3.0.CO;2-#
web of science category Biochemistry & Molecular Biology; Biophysics
subject category Biochemistry & Molecular Biology; Biophysics
unique article identifier WOS:000089687600004
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journal analysis (jcr 2019):
journal impact factor 1.854
5 year journal impact factor 1.909
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