abstract
This paper describes a metabonomics study of 2nd trimester biofluids (amniotic fluid, maternal urine, and blood plasma), in an attempt to correlate biofluid metabolic changes with suspected/diagnosed fetal malformations (FM) and chromosomal disorders as well as with later occurring gestational diabetes mellitus (GDM), preterm delivery (PTD), and premature rupture of membranes (PROM). The global biochemical picture given by the threesome of biofluids should enable the definition of potential disease signatures and unveil potential metabolite markers for clinical use in predictive prenatal diagnostics. Results show that relatively strong metabolic disturbances accompany FM, reflected in all three biofluids and thus suggesting the involvement of both fetal and maternal metabolisms. Regarding GDM, amniotic fluid and maternal urine seem potential good media to detect early metabolic changes, and PTD subjects show small metabolite changes in the same biofluids, undergoing work being focused on plasma composition. Chromosomal disorders show an interestingly marked effect on maternal urine, whereas no statistically relevant early changes have been observed for PROM subjects. Interestingly, in the case of FM and chromosomal disorders, maternal biofluids show some sensitivity to disorder type, for example, for central nervous system malformations and trisomy 21, respectively. These results show the usefulness of biofluid metabonomics to probe overall metabolic disturbances in relation to prenatal disorders.
keywords
NUCLEAR-MAGNETIC-RESONANCE; TRIMESTER AMNIOTIC-FLUID; PRENATAL DISORDERS; NMR-SPECTROSCOPY; MATERNAL URINE; BIOMARKERS; 2ND; METABONOMICS; PREECLAMPSIA; PLASMA
subject category
Biochemistry & Molecular Biology; Spectroscopy
authors
Graca, G; Diaz, SO; Pinto, J; Barros, AS; Duarte, IF; Goodfellow, BJ; Galhano, E; Pita, C; Almeida, MD; Carreira, IM; Gil, AM
our authors
Ana Maria Pissarra Coelho Gil
Associate Professor with Aggregation
António de Sousa Barros
Post-doc Fellowship
Brian James Goodfellow
Assistant Professor
Iola Melissa Fernandes Duarte
Principal Researcher
Joana Isabel Monteiro Pinto
Post-doc Fellowshipacknowledgements
Funding is acknowledged from the European Regional Development Fund through the Competitive Factors Thematic Operational Programme and from the Foundation for Science and Technology (FCT), Portugal (research Project PTDC/QUI/66523/2006 and research Grants SFRH/BD/41869/2007 and SFRH/BD/64159/2009). The Portuguese National NMR Network (RNRMN), supported with FCT funds is also acknowledged, and the authors are grateful to M. Spraul, Bruker BioSpin, Germany, for providing access to spectral NMR databases.