resumo
Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures. In this work, the extraction ability for bovine serum albumin (BSA) of aqueous biphasic systems (ABS) formed by polypropylene glycol 400 (PPG 400) and several GB-ILs was evaluated. ABS formed by PPG 400 and cholinium chloride ([Ch]Cl), GBs, and sucrose were also investigated for comparison purposes. It is shown that BSA preferentially migrates for the GB-IL-rich phase, with extraction efficiencies of 100%, achieved in a single-step. Dynamic light scattering, and circular dichroism (CD) and Fourier transform infrared (FTIR) spectroscopies were employed to evaluate the effect of the investigated cholinium-based GB-ILs on the BSA stability, and compared with results obtained for the respective GBs precursors, [Ch]Cl and sucrose, a well-known protein stabilizer. Molecular docking studies were also carried out to investigate on the binding sites of GB-IL ions to BSA. The experimental results confirm that BSA has a higher stability in GB-ILs than in any of the other compounds investigated. (C) 2015 Elsevier Ltd. All rights reserved.
palavras-chave
AQUEOUS 2-PHASE SYSTEMS; PROTEIN SECONDARY STRUCTURE; BIPHASIC SYSTEMS; BIOLOGICAL-RESEARCH; ALPHA-CHYMOTRYPSIN; SEPARATION; MEDIA; CHROMATOGRAPHY; PARTITION; EFFICIENT
categoria
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
autores
Taha, M; Quental, MV; Correia, I; Freire, MG; Coutinho, JAP
nossos autores
Mara Guadalupe Freire Martins
Investigador Coordenador
Maria João Quental
Bolseiro de pós-Doutoramento
Mohamed Taha Ahmed Amin
Bolseiro de pós-DoutoramentoGrupos
G1 - Materiais Porosos e Nanossistemas
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
Projectos
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2013)
Igy Technology: A Purication Platform using Ionic-Liquid-Based Aqueous Biphasic Systems (IGYPURTECH)
agradecimentos
This work was developed in the scope of the project CICECO - Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013), financed by national funds through the FCT/MEC and co-financed by FEDER under the PT2020 Partnership Agreement. M. Taha acknowledges Fundacao para a Ciencia e Tecnologia (FCT) for the post-doctoral grant SFRH/BPD/78441/2011. M.G. Freire acknowledges the European Research Council (ERC) for the Grant ERC-2013-StG-337753. I. Correia acknowledges FCT for Investigator FCT contract.